Development, optimization and evaluation of polymeric electrospun nanofiber : as delivery of drugs for management for eye infection and inflammation.

Donya Barfar,1,* Shahla mirzaeei,2

1. Student Research Committee, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
2. Nano Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Introduction

Ophthalmic antibiotics are used to treat ocular infections including blepharitis, conjunctivitis, keratitis and several others. there are ophthalmic antibiotics available from a variety of drug classes including aminoglycosides, macrolides, polypeptides, quinolones and sulfonamides.1 in addition, many are available as combination products with other antibiotics or corticosteroids. blepharitis is a chronic inflammatory condition of the eyelids, often presenting with the symptoms of eye irritation and redness. overgrowth of normal bacterial flora plays a role in the pathophysiology of blepharitis.the goal of this study is to create a biodegradable nanofibre that can easily be seen and able to deliver the therapeutic concentrations of gentamicin and methylpradenizolone to treat infection and inflammation of the eye.

Methods

Poly e-caprolactone (pcl) nanofibers containing gentamycin methylpradenizolone were successfully electrospunand evaluated for infection and inflammation of the eye. pcl , gentamicin and methylpradenizolonein mixtures of dimethylformamide:aceton with ratios of 1:1 v/v were prepared, and the nanofibers were produced by electrospinning technique. scanning electron microscopy (sem) was used to investigate the morphology and average diameter of the electrospun nanofibers.

Results

Nanofibers had a smooth and bead-less morphology with the diameter ranging from 175 to 320 nm. dsc results indicated dispersion of gentamicin and methylpradenizolonein in the pcl nanofibers and showed a decrease in crystallinity of pcl nanofibers by adding gentamicin and methylpradenizolonein. in vitro drug release studies in phosphate buffer solution (ph 7.4) showed that the drug release rate was affected by the solvents ratio and the drug concentration. sustained drug release was prolonged to 15 days.

Conclusion

The prolonged drug release, together with the proven biocompatibility, antibacterial and anti inflammation mechanical properties of drug-loaded core shell nanofibers, makes them a promising candidate for use as a drug delivery system to treat infection and inflammation of the eye.

Keywords

Nanofibers , antibiotics , electrospun , infection , inflammation , pcl