Chemical composition and antifungal activity of nectaroscordum tripedale extract against some pathogenic dermatophyte strains

Zahra Chegeni,1 Behrouz ezatpour,2 Asghar sepahvand,3 Mojtaba mirshekari sloeimani,4,*

2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
4. Bam University of Medical Sciences, Bam, Iran

Abstract

Introduction

Diseases caused by fungi belonging to the three main genera of microsporum spp, trichophyton spp and epidermophyton floccosum are called dermatophytosis; which caused significant medical and health problems in all countries around the world. currently, the use of chemical treatments available for this disease is limited because of their high toxicity, as well as the occurrence of some drug resistance. this investigation aims to assess the chemical composition and antidermatophytic properties of nectaroscordum tripedale against some pathogenic dermatophyte strains.

Methods

In vitro antidermatophytic effects of n. tripedale extract on trichophyton mentagrophytes, microsporum canis, and microsporum gypseum was determined using the determination of minimum inhibitory concentration (mic) and minimum fungicidal concentration (mfc) based on the broth microdilution method, according to the protocol m38-a2 of the clinical and laboratory standards institute (clsi) for filamentous fungi with some modifications. the components of the n. tripedale extract were identified by gas chromatography/mass spectroscopy (gc/ms) analysis.

Results

The results demonstrated that n. tripedale extract had both fungistatic and fungicidal activities with the mic and mfc ranging from 16.6 to 66 mg/ml. the main compounds are decadienal (11.1%), hexadecanoic acid (10.3%), and heptadecane (9.5%), respectively.

Conclusion

Obtained results of this investigation recommend a first step in the search of new antidermatophytic agent and support the use of n. tripedale in the folk medicine for dermatophytic infections.

Keywords

Gc/ms, microsporum canis, microsporum gypseum, trichophyton mentagrophytes, in vitro