Evaluation of the effect of type 2 diabetic serum on malondialdehyde, superoxide dismutase and catalase activity in hdf cancer cell line compared to normal human fibroblast cells

Farzane Hasanzade,1 Safoura sameni ,2,* Seyedeh sara hashemi ,3 Hanie jafary ,4

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3. Burn & Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Introduction cancer is one of the most important health problems and a main common cause of mortality in the world. therefore, its prevention, diagnosis and treatment are of interest to many researchers in biological sciences. diabetes is one of the most common chronic diseases showing a growing trend in the global statistics including iran. type 2 diabetes and cancer have many common risk factors, but the potential biological links between the two diseases are not fully understood yet. evidences obtained from studies by different researchers suggest that increased blood glucose or hyperglycemia in diabetes triggers the release of free radicals and stimulates glycosylation of free radicals’ inhibitor enzymes. some other studies indicate that some hyperglycemic drugs are associated with increased/decreased risk of cancer. in addition, some other studies have shown that increased oxidative stress induction (imbalanced production of free radicals and antioxidant immune system) can be used as a treatment for cancer. target this study aims to evaluate the effect of serum of patients with type 2 diabetes on oxidative stress changes in hdf cancer cells and its comparison with human fibroblast cells. the importance of research the discovery of the relationship between cancer and diabetes as two common diseases in the general populations is crucial for timely, rational and informed decision-making, not only in the field of public health and social economy, but also for the purposeful prevention and managing of diabetes in everyday clinical practice. the serum of diabetic patients contains a high level of harmful molecular factors for the cells. increased blood sugar or hyperglycemia in diabetes triggers the production of free radicals and glycosylation of free radical inhibitor enzymes. on the other hand, studies have shown that increased induction of oxidative stress can be used as a treatment for cancer. in this study, for the first time, the inhibitory effect of these factors in serum of diabetic patients is investigated on oxidative stress induction in cancer cells.

Methods

Total amount of 20cc blood samples were taken from one selected (based on specific criteria) diabetic patient and one healthy individual based on the ethics charter of the shiraz university of medical sciences, and ultimately their blood serum was extracted. in this study, hdf cell line and human fibroblast cells were cultured in dmem medium. the serum of diabetic and normal individuals and fbs serum (as control) were then added to the culture medium, separately. after 24 hours, the cellular supernatant liquid was collected, and afterwards, the oxidative stress parameters were measured based on the activity of cat, mda and sod.

Results

The exact results of the measured parameters indicate that the culture medium containing type 2 diabetes serum has a greater effect on the levels of cat, mda, sod enzymes produced by cancer cells than the culture medium containing the fbs serum. furthermore, in cancer cells the level of these enzymes is higher than the human fibroblast cells.

Conclusion

According to our findings, the level of cat, mda and sod enzymes in cancer cells has increased in comparison with normal fibroblasts. therefore, these enzymes, which cause the oxidative stress by increasing the level of free radicals, can also lead to the destruction of cancer cells, and thereby, they can be considered as an effective factor in healing the cancer

Keywords

Oxidative stress - cancer - diabetes