Effect of curcumin based electrospun nanofibrous scaffolds on proliferation and stemness preservation of adipose derived stem cells

Samira Mashayekhi,1 Mohammad pourhassan-moghaddam,2,* Maryam ghobeh,3

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Department of Medical Biotechnology, Tabriz University of Medical Sciences, Tabriz ,Iran
3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction

An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. curcumin (cur) is a naturally occurring flavone with a wide spectrum of biological functions including anti-inflammatory and anti-oxidant properties. the present study describes the influence of cur-loaded nanofibrous mats on the regulation of proliferation and stemness preservation of adipose-derived stem cells (adscs).

Methods

For this purpose, cur-loaded poly (e-caprolactone)/poly (ethylene glycol) (pcl/peg) nanofibrous mats were produced via electrospinning process and the successful fabrication of these bioactive mats was confirmed by field emission scanning electron microscopy (fe-sem) and fourier transform infrared spectroscopy. adscs were seeded on the nanofibers and their morphology, viability, and stemness expression were analyzed using fe-sem, mtt, and qpcr assays after 2 weeks of incubation, respectively.

Results

The results display that adscs exhibit better adhesion and significantly increased viability on the chr loaded pcl/peg nanofibrous mats in relative to the pcl/peg nanofibers and tissue culture polystyrene. the greater viability of adscs on cur based nanofibers was further confirmed by higher expression levels of stemness markers sox-2, nanog, oct-4, and rex-1.

Conclusion

These findings demonstrate that cur-loaded pcl/peg electrospun nanofibrous mats can be applied to improve cell adhesion and proliferation while concurrently preserving the stemness of adscs, thus representing a hopeful potential for application in stem cell therapy strategies.

Keywords

Curcumin, electrospun nanofibers, stem cells, pluripotency