1. Department of Biology, Faculty of Basic Sciences, SCIENCE AND RESERCH Branch, Islamic Azad University, Tehran, Iran 2. Department of Pathology, Gastrointestinal Cancer Research Center, Imam Hospital, Mazandaran University of Medical Sciences, Sari, Iran 3. Department of Internal Medicine Mazandaran University of Medical Sciences Sari, IRAN
Abstract
Introduction
Gastric cancer is the second most common cancer after lung cancer in the world. the mutation in the kras gene is one of the causes of resistance to chemotherapy and radiotherapy in patients with cancer such as gastric cancer. the aim of this study was to evaluate the frequency of mutations in kras gene (codon 12 and 13) in patients with gastric cancer in northern iran.
Methods
This study was a case-control study including patients with gastric cancer who referred to imam khomeini hospital in sari during the years 2009 to 2015 and were treated. the pcr-rflp method was used in this study. the statistical analysis was performed using spss statistical software on 100 samples of cancer tissue from gastric cancer patients plus 50 samples of healthy tissue obtained from the surgery or endoscopy of the same patients.
Results
There were no cases of mutations in the kras gene (codon 12 and 13) from 100 samples from gastric cancer patients and 50 healthy tissue samples from the same patients
Conclusion
The review and analysis of the mutations of kras gene (codon 12 and 13) in more number of samples using other methods can be helpful in helping the diagnostic and therapeutic process of patients suspected of gastric cancer.
