Changes in expression of survivin anti-apoptotic gene under treating with cyclophosphamide after 24 hours in mcf-7 cell category of breast cancer

Somayeh Gholami,1,* Dr.golnaz asaadi tehrani,2 Fateme modhejiyan,3 Narges mohammadi,4

1. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
2. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
3. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
4. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran

Abstract

Introduction

Breast cancer is the most common type of cancer among women. one of the genes involved in breast cancer is survivin, which is an apoptosis inhibitor protein (iap) and plays an important role in regulation of cell cycle and apoptosis. distinct expression of this gene in tumor cells compared with normal mature cells, and high expression of this gene in malignant tumors has highlighted it as the fourth major transcriptum in tumors and also as a biomarker. cyclophosphamide is one of the most commonly drugs used for breast cancer chemotherapy. cyclophosphamide is an anti-neoplasm, an alkylated and an inhibitor of dna replication. therefore it is important to study expression of this gene under cyclophosphamide treatment.

Methods

Human breast tumor cell lines (mcf-7) were prepared and cultured from the iranian institute of pasteur cell bank, and after counting and passage, treated with cyclophosphamide at different concentrations of 1, 5 and 10 μm and then the effect of the drug over a period of 24 hours was investigated for observing the survivin gene expression changes. rna extraction and cdna synthesis were performed using roche and takara kits, respectively then by optimizing the real time pcr condition, survivin gene expression was studied and finally the data was analyzed by using rest software.

Results

According to studies, considering the fact that survivin is an anti-apoptotic gene, and high expression of survivn escalate breast cancer, the results indicate that expression of this gene after 24 hours of treatment with cyclophosphamide significantly decreased (p = 0.001), which can be an important factor in improving the disease.

Conclusion

The study of the effect of taxol on the survivin gene expression suggests that the medicine regulates the gene for apoptosis in breast cancer cells, which actually reduces expression of survivin. in this study, the results also indicated that cyclophosphamide treatment of 24 hours reduces the expression of survivin and apoptosis in cancer cells. by investigating the survivin gene expression under the cyclophosphamide treatment, early detection of cancer, more targeted chemotherapy for cancer control and accelerated treatment of this disease can be achieved.

Keywords

Breast cancer, survivin, cyclophosphamide, rest