The effect of probiotic lactobacillus plantarum isolated from traditional semnan cheese on lipid profile and hyperglycemia of streptozotocin-induced diabetic wistar rats

Mahnoosh Parsaeimehr,1,* Mahmood ahmadi hamedani,2 Sahar mosallemi,3

1. Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
2. Clinical Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
3. Graduate Student, Faculty of veterinary medicine, Semnan university,Semnan

Abstract

Introduction

Diabetes is one of the most important metabolic disorders that is dramatically increasing and can cause many health complications. so probiotics can be considered as a natural and safe solution to control and delay such complications. the effect of probiotic lactobacillus plantarum isolated from traditional semnan cheese on lipid profile and hyperglycemia of streptozotocin-induced diabetic rats was evaluated.

Methods

In this study 24 male wistar rats were randomly divided into four groups (six rats in each group) as given below. group1: normal control rats, received normal saline (10 ml/kg), group 2: diabaetic control rats received normal saline (10 ml/kg), group 3: diabaetic rats received l.plantarum (2 cc/day.), group 4: normal rats received l.plantarum (2 cc/day). isolation and identification of lactobacillus plantarum from traditional semnan cheese were performed through biochemical tests and 16s rdna sequencing. hyperglycemia was induced in male rats by intraperitoneal injection of streptozotocin (65mg/kg). all treatments were orally administered to rats for 21 days .fasing blood sugar (fbs) was monitored at 0, 7, 14 and 21 days after induction of diabetes, lipid profile of blood serum of heart killed rats were measured at the end of experiment in 21th day. statistical differences among groups were analyzed using one-way analysis of variance (anova) followed by tukey’s multiple tests.

Results

The results of this study showed that lactobacillus plantarum suspension with 108 cfu/ml doses in treated diabetic rats significantly (p> 0.05) decreased fasting blood glucose and triglyceride compared to the control group.

Conclusion

According to the results of this study, it can be deduced that consumption of probiotic lactobacillus plantarum can be effective in improving biochemical parameters due to the destructive effects of diabetes mellitus and moreover, as a preventive measure and as a new auxiliary treatment approach for diabetic patients can be considered. however, more clinical studies are needed to prove the exact effect of this probiotic on diabetes.

Keywords

Diabetes, probiotic, hyperglycemia, lipid profile