Evaluation of expression of mir-20a and mir-30a in helicobacter pylori infected patients compared to gastric cancer patients by real time pcr technique
Gastric cancer is one of the most common cancers in the world and the second leading cause of death in the world after lung cancer. one of the main causes of this disease is the helicobacter pylori bacteria. also, mirnas are effective in most biological processes. the objective of this study is to evaluate the mir-30a and mir-20a expression in the patients infected helicobacter pylori compared with gastric cancer patients.
Methods
For this purpose, the real time pcr technique was used. in this study, samples were selected from through the patients referred to imam khomeini and shariati hospitals. the population was 40 people who had cancer but they were not under any treatment, 40 patients infected with helicobacter pylori who were referred to the hospital for other treatments, but they did not have cancer, and 12 people who were not infected with helicobacter or gastric cancer.
Results
The findings of the study indicated that changes of mirna-30a, mirna- 20a expression in the blood of patients infected helicobacter pylori and gastric cancer are higher than healthy people who are also negative in terms of helicobacter pylori, and comparatively, changes of mir-30, mirna-20a expression in the blood of individuals who are healthy but in terms of h. pylori are positive were lower than the previous two groups.
Conclusion
Based on the results helicobacter pylori can enhance the expression of mir-20a and mir-30a in patients with gastric cancer.and these mirnas play an important role in the induction and pathogenesis of gastric cancer. we can also use micro-rnas as biomarkers to diagnose the disease.
Keywords
Gastric cancer, helicobacter pylori bacteria, real time pcr, mirnas
