Investigation of the properties of molybdenum nano-composites modified with hepatitis b surface anti-antigens antibody

Zeinab Solimani,1 Mostafa shourian,2,*

1. University of Guilan
2. University of Guilan

Abstract

Introduction

Hepatitis b is one of the factors that causes damage to the liver and even leads to death, as well as in both chronic and acute forms. nanoparticle compounds are used to enhance detection of surface antigens of the virus. nanoparticle compounds, due to the properties of increased levels relative to the volume and the accumulation of energy levels, and other proper characteristics of this work. the molybdenum nanocomposites contain heavy metals with magnetic properties, and are easy to propagate in water and are easy to synthesize. the use of fluorescence makes it easier to detect antigens. the fluorescence-based diagnostic methods are one of the techniques that can have a high sensitivity to detect surface antigen of hepatitis b virus.

Methods

Molybdenum nanocomposites have been used to study the hepatitis b surface antigen. molybdenum nanocomposites were studied using uv-vis and fluorescence spectroscopy, ftir, dls and zeta potential. after that, the binding of antibodies to the surface of the molybdenum nanocomposites was carried out through covalent bonding and adsorption. in this method, molybdenum nanocomposites were synthesized from the desired salts and after dispersing in water, then, by dls and zeta-potentials tests, their size and precipitation were determined. in the next step, the antibody against the surface antigen of hepatitis b was bonded onto the surface of the nanoparticles, which can be accomplished through adsorption or covalent bonding.

Results

molybdenum nano-composites modified with hepatitis b surface anti-antigens antibody have been studied by the devices mentioned above, which indicate that fluorescence is present in this type of molybdenum nanocomposites. this same factor makes it possible, after binding of the antibody to the molybdenum nanocomposites, to use these as nano-libels to detect surface antigen of hepatitis b virus. based on, the fluorescence spectra of these molybdenum and antibody nano-composites, as well as the mix of these two, have been studied and the ability of these molybdenum nano-composites in the production of the signal for diagnosis has been proven. these 595nm molybdenum nanocomposites and antibodies are also in the range of 330-450nm, and their mixing is less pronounced at 345nm and 595nm wavelengths. also, ftir studies confirm the binding of antibodies to this molybdenum nanocomposite.

Conclusion

These molybdenum nano-composites modified with hepatitis b surface anti-antigens antibody have the ability to conjugate with antigen, and their detection by the fluorescence machine is easily possible. in fact, these molybdenum nano-composites are used as an immunonano-libel in biosensors, and in fact, this method is commercially important for early diagnosis and early prevention of this disease. it also has a significant advantage over the absorption spectrum and elisa test.

Keywords

Fluorescence spectroscopy, nano-libels, anti-hepatitis b antibody, hepatitis b virus antigens