Effects of resveratrol on foxo1 and foxo3a gene expression in adipose tissue, and serum insulin, insulin resistance and serum sod activity in the type 2 diabetic rats

Hosein Mohammadi,1,* Yaser mohassel,2 Sonia salari,3

1. Department of Clinical Biochemistry, Faculty of medicine, Kermanshah University of Medical Sciences, Kermanshah- Iran
2. Department of Clinical Biochemistry, Faculty of medicine, Kermanshah University of Medical Sciences, Kermanshah- Iran
3. Department of Clinical Biochemistry, Faculty of medicine, Kermanshah University of Medical Sciences, Kermanshah- Iran

Abstract

Introduction

Diabetes mellitus (dm) is a common metabolic disorder characterized by elevated blood glucose level. oxidative stress plays a critical role in insulin resistance. fork head-related transcription factor (foxo) proteins are important transcriptional factors involved in oxidative stress and insulin resistance. resveratrol (rsv) is a polyphenol with hypoglycemic and antioxidant properties.

Methods

The aims of present study were to examine the effects of rsv on foxo gene expression, serum superoxide dismutase (sod) activity, insulin level, and insulin resistance in type 2 diabetic (t2dm) rats. thirty male wistar rats were used in this study. dm was induced in rats (n=24) using streptozotocin (stz) & nicotinamide (na); then, they were divided into 4 groups (each group with 6 rats). six untreated normal rats were used as normal control group (group 1); diabetic rats in groups 2 to 5 were treated by 0, 1, 5 and 10 mg /kg body weight of rvs, respectively for 30 days. at the end of experimental period, the rats were sacrificed, abdominal incision was given and blood was collected from the cardiac puncture, serum was separated, adipose tissues was also obtained and stored at −80°c. glucose was determined by pars azmun kit (iran). insulin serum level was determined by elisa method. sod activity inserum was determined using randox kit(england).

Results

The foxo1 and foxo3a genes expression in adipose tissue were evaluated using real‐time pcr. the p<0.05 was considered as a significant level. findings of the present study indicated that resveratrol significantly reduced blood glucose level and increased insulin level and improved the insulin sensitivity. resveratrol resulted in an increased serum sod activity and caused decreased expression of foxo1 and foxo3a in adipose tissue of rats with t2dm.

Conclusion

The results showed that the rvs by attenuation of foxo expression in adipose tissue of type 2 diabetic rats had the hypoglycemic potential and antioxidant properties and consequently ameliorated insulin resistance.

Keywords

Diabetes mellitus, oxidative stress, foxo, superoxide dismutase