The effect of amniotic membrane-derived mesenchymal cells transplantation on cardiac function improvement in a model of heart failure in male rats through the effect on the expression of bcl2, bax a
1. iran university medical sciences 2. iran university medical sciences
Abstract
Introduction
The effect of amniotic membrane-derived mesenchymal cells transplantation on cardiac function improvement in a model of heart failure in male rats
through the effect on the expression of bcl2, bax and p53 proteins
Methods
In this study, 35 male wistar rats were randomly assigned into 5 groups ( 7 in each group). study groups were: 1. control group, 2. heart failure group (hf), 3. sham group, 4. culture media group, 5. stem cells transplantation group (sct). heart failure model was induced by subcutaneous injection of 170 mg/kg/d isoproterenol during 4 consecutive days and the induction was confirmed by echocardiography 28 days later. in stem cells transplantation group, 3 × 106 cells were transplanted into myocardial tissue in a 150 µl culture media. by use of echocardiography, cardiac function was examined. western blot method was used to determine the expression of bcl2, bax and p53 proteins.
Results
: echocardiography parameters showed that ejection fraction in hf group increased from 58.73± 0.9 to 81.25± 0.5 in the sct group which indicates 22.52 change in this parameter. fractional shortening increased from 27.8±53.58 in hf group to 45.6±55.91 in the sct group indicating a change of 22.52. protein level measurements showed increased levels of p53 and bax proteins in the hf group
Conclusion
: it seems that amniotic membrane-derived mesenchymal stem cells transplantation significantly improves the cardiac function in heart failure through effects on the expression of bcl2, bax and p53 proteins.
