Sepsis is a life-threatening condition caused by the uncontrolled, systemic, inflammatory response tobacterial, viral or fungal infections. rapid and accurate identification of sepsis and its causative organisms are necessary. the aim of this study was design and evaluation of multiplex pcr to identify of the most common pathogenic bacteria involved in septicemia.
Methods
This cross-sectional study was performed in isfahan university of medical science, isfahan, iran, genetic targets for primer designing included the16s rdna, rpob,gyrband x chromosome (as internal control). the sensitivity and specificity of the multiplex pcr are elevated by standard strains. one hundred and twenty-six
samples collected from alzahr a hospital were tested
by multiplex pcr.
Results
The multiplex pcr showed sensitivity ranging from 1 to 100 target gene
copies per reaction (or 50-100 cfu/ml) depending on the bacterial
species. of the 126 samples 39 samples were positive. a good correlation was found between the results obtain ned by the two methods. the results obtained by pcr were identical to those obtained by conventi onal methods in 26 out of 39 cases. of the remaining 13 samples, 8 (6.34%) samples were detected only by pcr and 5 (3.96%) samples were identified only by blood culture.
staphylococcus aureus and escherichia coli were the most prevalent bacteria
which isolated and identified in blood culture of hospitalized patients in the intensive care unit. the sensitivity and specificity of the multiplex pcr were 83.87% and 91.58% respectively.
Conclusion
The presented multiplex pcr permits a rapid and accurate detection of some pathogenic bacteria in blood samples which causes successful therapy in hospitalized patients. in addition to its use in blood samples, the multiplex assay may be value for the detection of these bacteria in other clinical samples.
