Study of 40-bp insertion/deletion polymorphism of murine double minute2 (mdm2) and the risk of colorectal cancer in iranian population

Mohammad Mohammadi,1,* Ladan kiani,2 Mohammad reza hajari,3

1. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Introduction

Mouse double minute 2 homolog (mdm2) is an important negative regulator of the p53. p53 plays a central role in the cell cycle regulation mechanisms and cell proliferation control, and its inactivation is considered a key event in carcinogenesis. overexpression of mdm2 gene has been reported in several human tumors. increasing the expression level of mdm2 protein due to genetic changes in its promoter region leads to a decrease in the activity of p53 protein. in this study, we aimed to evaluate the effect of 40-bp insertion/deletion (ins/del) polymorphism on the promoter of mdm2 and susceptibility to colorectal cancer in a sample of iranian population.

Methods

This study was carried out on 120 patients with colorectal cancer and 120 healthy individuals. genomic dna was extracted from the whole blood by the salting-out method. the 40-bp ins/del polymorphism was determined by using pcr.

Results

The results of genotypic study (or = 1.266, 95% ci = 0.7244-2.214, p = 0.477 for i / d and or = 1.662, 95% ci = 0.7183- 3.847, p = 0.2928 for d / d) and allelic study (or = 1.341, 95% ci = 0.8966-2.006, p = 0.1838) showed no significant difference between the affected individuals and control group.

Conclusion

The results of this study showed that there is no association between 40-bp ins/del polymorphism in the promoter of mdm2 and the incidence of colorectal cancer in iranian population.

Keywords

Mdm2, polymorphism, colorectal cancer