Determination of gut microbiota pattern in cvd patient in comparision with healthy control in irainian population

Seyedeh fatemeh Sadati khalili,1 Maryam taj abadi ebrahimi,2,* Seyed davar siadat,3

1. The Islamic Azad University, Central Branch
2. The Islamic Azad University, Central Branch
3. Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran

Abstract

Introduction

Cardio vascular diseas (cvd) is the third most common cause of death in most countries. atherosclerosis (at) ,is defined as increased thickness of artery endothelium due to fat accumulation, is major cause of cvd. at has various pathology including genetic and environmental factors. it has been demonstrated that gut microbiota as environmental factor has significant role in induction and development of at. the gut microbiota is microbial community colonizes the human gastro intestinal track. gut microbiota composition consist of various microorganism including bacteria, protozoa, fungi, archea and virus. gut microbiota pattern has determinative role on health and disease state in the host. in this regard, relative abundance of important microbiota members (a. muciniphila, lactobacillu and prevotellaceae) were determined in cvd patient and control in irainian population for the first time.

Methods

15 cvd patients and 15 healthy subject recruited from november 10, 2016 to august 28, 2018 in tehran. lipid profiles (cholesterol, triglyceride, ldl, hdl, vldl) and acute phase protein (crp) were measured by biochemical tests and saa were measured by elisa kit (ab100635 – serum amyloid a (saa) human elisa kit) respectively. in order to determine the relative abundance of a. muciniphila and lactobacillus and prevotellaceae, stool samples were collected. following dna extraction from samples by dna-extraction qiaamp® dna stool mini kit (50), quantitative pcr was conducted based on 16s rdna universal primers. the bacterial abundance was calculated based on standard curve derived from escherichia coli standard strain. finally, the frequency of these bacteria calculated. data of biochemical test and qpcr were analyzed by spss software and independent sample t test and data analysis of crp test was performed using mann-whitney test.

Results

Our data demonstrated that cholesterol, triglyceride, crp and saa of cvd group was higher compared with contrrol group. ldl and hdl of control group was higher compared with cvd group. ), no significant change obserserved in vldl test. although we we reported abundance change of a. muciniphila (p=0.248) and prevotellaceae (p=0.183) in cvd group compared with control group but this result is not significant. the relative abundance of lactobacillus was significantly decreased (p=.032) in cvd group compared with control group.

Conclusion

In conclution cvd patient has significantly different relative abundance of lactobacillus compared with control group. according to anti-inflamatory properties of lactobacillus our result is parallel with the lipid profile and acute phase protein (crp and saa). since reduction of lactobacillus relative abundance is parallel with lipid profile and acute phase protein (crp and saa), this could be explained anti-inflamatory properties of lactobacillus in gut microbiota host interaction. determination of important of gut microbiota in cvd patient could be promissing in control and treatment of at at a certain population.

Keywords

Microbiota, cardiovascular disease, lipid profile, crp, saa