Bioinformatics evaluation of hsa-mir-19a-3p related to single nucleotide polymorphism (rs200409468) of ptgs2 gene in patients suffering from gastric cancer

Sajede Naghiyan fesharaki,1,* Sajad naghiyan fesharaki,2

1. Department of Laboratory sciences, Faculty of Basic Science,Babol Branch,Islamic Azad University,Babol,Mazandaran,Iran
2. Department of Laboratory sciences, Faculty of Basic Science,Babol Branch,Islamic Azad University,Babol,Mazandaran,Iran

Abstract

Introduction

Gastric cancer has been known as the second cause of mortality in consequence of cancer . this cancer can make many problem for families and societies and also this is a good reason for recognizing biomarkers. ptgs2 gene is located on choromosome position of . the surveys show that this gene is concerned with gastric cancer.based on accomplished studies, expression of this gene may be a prognostic indicator foe several type of cancer. single nucleotid polymorphisms are consisting genetic marker related to many of the genetic disease. micro rnas are counted as bio- markers with regulation and control of genes expression at level of mrna. genes that enforce their effects. our aim is assessment of bioinformatics analysis of hsa-mir-19a-3p concerned with single nucleotide (rs200409468) of ptgs2 gene and suffering from gastric cancer.

Methods

That is why databases such as ncbi , mirnasnp , mirwalk 2.0, phemomir, mirbase and david have been used.

Results

Due to the studies in rs200409468, have determined the position of this snp, t allele to c conversion, that caused possible effected on function and connection of micrornas related to this area. the performed studies on hsa-mir-19a-3p have determined that , this microrna with a high level of effect on 3’utr, is a subscript of ptgs2 connection.

Conclusion

Regarding to this confirmation of the role of hsa-mir-19a-3p in gastric cancer done by the aid of data bases for example phenomir, acquiring target gene by the aid of mirwalk2.0 database and assessment of signaling pathways concerned with use of david database, this micrornas has been choosen for next analysis stages. according to this, t allele in this snp location can be related with gastric cancer.

Keywords

Single nucleotide polymorphisme (rs200409468), ptgs2 gene, microrna (hsa-mir-19a-3p), gastric cancer