Oral administration of salvia hydrangea extract (an iranian sage) nrf2 in rat model of alzheimer’s disease

Rana Sirous azar,1 Afshin kheradmand,2 Solmaz khalifeh,3,* Mahshid shaabanpouremam,4

1. Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University
2. Department of pharmacology, school of pharmacy-international campus, Iran university of medical sciences, Tehran, Iran
3. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University
4. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University

Abstract

Introduction

Alzheimer’s disease (ad) is the world’s most familiar dementing illness. neurodegenerative disorders are generally characterized by abnormal aggregation and deposition of specific proteins. amyloid beta (aβ) plaques are one major characteristic of ad pathology. aβ originates from proteolysis of the amyloid precursor protein and is the leading trigger of neurotoxicity and oxidative damage in ad

Methods

We used ad models of rats by injecting aβ to determine the nrf2 protein level by orally administration of s.hydrangea in the hippocampus and frontal cortex of male wistar rats. salvia genus is a well-known traditional chinese herbal medicine that is widely used in the clinical treatment of different diseases. in a period of ten day, two groups of rats were fed with s.hydrangea in concentration of 50mg/kg/day by oral gavage syringe directly. in this study, animals were divided into four groups: vehicle group; which received oral gavage of water (1 ml) daily for 10 days before the hippocampal injection of 3 µl pbs. aβ injected group, received oral gavage of water (1 ml) for 10 days before the injection of aβ (10 ng/µl). two other groups pretreated by 1 ml solution of s. hydrangea every day for 10 days (50 mg/kg), then received aβ and/or pbs in ca1 region.

Results

We have found that nrf-2 levels were increased about 1.3 fold in hippocampus and about 1.2 fold in frontal cortex in aβ injected rats in compare with control group. in pretreated rats with s.hydrangea cause more increase in nrf2 factor about 1.2 fold in hippocampus and 1.25 fold in frontal cortex in compare with aβ-injected rats.orally treatment of s.hydrangea extract in aβ-injected rats could change nrf2 protein level which is master regulator of antioxidant defense system.

Conclusion

In summary, orally treatment of s.hydrangea extract in aβ-injected rats could nrf2 protein level. for these reasons, s.hydrangea extract and its constitutive compounds can be one of therapeutic drugs and prevent neurodegeneration in rat ad model.

Keywords

Salvia hydrangea, amyloid beta, nrf2