Evaluation of anticancer effects of lactobacillus paracasei sp. st1 isolated from azerbaijan traditional dairy product on colorectal tumor cells hct116
1. Department of Biology, Faculty of Natural Science, University of Tabriz 2. Department of Biology, Faculty of Natural Science, University of Tabriz 3. Department of Biology, Faculty of Natural Science, University of Tabriz
Abstract
Introduction
Today, many of the natural health products have attracted the attention of scientists. natural health products may have anticancer effects due to the probiotic activity of their existing organisms. lactic acid bacteria are the most important group of microorganisms used in food fermentation, that among them, the genus lactobacillus is a very significant microorganism. there is evidence that the use of lactobacillus species reduces the risk of cancer and these bacteria have anticancer effects. this project was carried out with a focus on reviewing the anti-cancer activity of lactobacilli in the "shoor" traditional dairy product.
Methods
In this study, shoor samples were collected from the region of azarbayjan and the suspensions were cultured in mrs agar medium. the isolates were identified by biochemical and molecular methods. supernatant was extracted by mrs broth medium and its cytotoxicity was evaluated on hct116 cancer cells by microscopy and mtt assay.
Results
Cell assays results showed that supernatant of st1 isolate had a significant anticancer effect on cancer cell line (p<0.05). the results showed that the isolate was 99% compatible with lactobacillus paracasei.
Conclusion
Results achieved in this study indicate that lactobacilli in azerbaijan traditional dairy products can be suitable candidates to find effective anti-cancer compounds. paying attention to traditional dairy products and encouraging people to use probiotics, can be useful in increasing the health of the community. in addition, it is very important to study the application of these microorganisms as starter.
