Study of relation on breast cancer and a / t 251 polymorphism of il-8 gene in iranian female populations by tetra arms pcr

Elham Siasi,1,* Marzieh gholami ,2 Fatemeh ashrafi ,3

1. Ph.D, Department of Genetics, Faculity of science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2. M.Sc, Department of Genetics, Faculity of science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3. Ph.D, Department of Genetics, Faculity of science, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Breast cancer is one of the most important causes of minatory in women and interleukin 8 gene polymorphism (251 a / t) has a direct impact on the risk of breast cancer. therefore, in this study, was investigated the relationship between breast cancer and 251a / t polymorphism of il-8 gene in iranian females population by tetra arms-pcr.

Methods

In this case-control study, 50 women with breast cancer and 50 healthy women as control group were selected. after extraction of dna, determination of il-8 gene 251 a / t polymorphism genotypes was performed by tetra arms-pcr method.

Results

In control group, distributionof il-8 gene 251 a / t polymorphism frequency for aa, at, and tt genotypes was 0%, 88% and 12%, respectively and for cancer group was 12%, 54% and 34%, respectively. statistical analysis in this study showed no significant difference between aa, at and tt genotypes in the control and cancer groups (p> 0.05).

Conclusion

The present study is the first study for relationship between risk of breast cancer and the association of il-8 gene 251 a / t polymorphism in iranian women. the results were indicated that this polymorphism was not associated with breast cancer in this study female patients.

Keywords

Il-8 gene 251 a / t polymorphism, breast cancer, tetra arms-pcr technique.