Localized surface plasmon resonance (lspr) nanobioprobe for influenza a virus detection based on gold nanoparticles-anti nucleoprotein (np) conjugation

Mehri Mirkamali,1 Azadeh azizi,2,* Farida behzadian,3 Zeinab bagheri,4

1. Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
2. Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
3. Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
4. Faculty of Life Sciences and Biotechnology, Shahid Beheshti University G.C., Velenjak, Tehran, Iran

Abstract

Introduction

Nowadays, nanobiosensors are commonly used in order to detect wide variety of viral and bacterial agents. among them, nanobiosensors based on local surface plasmon resonance (lspr) of plasmonic nanoparticles provided a suitable base for the rapid diagnosis of various pathogenic agents. this property is due to the concentration of electric field at the surface of plasmonic nanoparticles. herein, a lspr based nanobioprobe has been designed in order to detect type a of human influenza virus. nanobioprobe has been fabricated based on the electrostatic conjugation between the specific antibody against the nucleoprotein (np) antigen of the virus and gold nanoparticles (gnps).

Methods

For this purpose, gnps-antibody conjugation has been confirmed by monitoring the lspr spectra and dynamic light scattering (dls) technique. after that, in order to investigate the lspr nanobioprobe sensitivity, the lspr band alterations of gnps have been monitored in the presence of different concentrations of influenza virus. finally, the sensitivity of the lspr nanobioprobe was compared with elisa technique.

Results

According to the transition electron microscopy (tem) images, the average diameter of gnps was about 40 nm and the dls results showed that the size of gnps has been increased after the conjugation with antibody. moreover, lspr bands alteration showed that the sensitivity of the nanobioprobe to detect the virus was 10 pfu/ml. however, based on the results, the sensitivity of the elisa method is much lower than the lspr method.

Conclusion

In conclusion, compared to the traditional techniques such as elisa, the sensitivity of the lspr nanobioprobe method is much more favorable for the rapid visual detection of pathogenic agents.

Keywords

Influenza virus; gold nanoparticles (gnps); local surface plasmon resonance (lspr); nanobioprobe