1. Environment Research Center, Research Institute for Primordial Prevention of NonCommunicable disease, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Introduction
Hydatid cyst is the larval stage of the tapeworm echinococcus granulosus.
hydatid cyst fluid, cyst membrane and protoscolices, contain a complex mixture of antigens
that can induce immune responses in the host. anti-cancer properties of protoscolices and
hydatid cyst fluid has been shown. in order to identify antigens of hydatid cyst fluid that have
anti-cancer effect, in this study production of monoclonal antibodies against one of the
hydatid cyst fluid band (40kda) that has cross reaction with sera of patients with breast
cancer has been investigated.
Methods
In this experimental study, 40kda band of hydatid cyst fluid was used as antigen.
a group of mice were immunized with this antigen, and then their spleen cells were extracted
and fused with sp2 cells. monoclonal antibodies production was checked in wells using
elisa and western blotting. the reaction of the produced monoclonal antibodies with breast
cancer cells was tested using flow cytometry method. finally effect of the monoclonal
antibodies on growth of breast cancer cells was investigated in vitro.
Results
The results of this study showed that monoclonal antibodies antibody against 40kda
were detected in several wells. the produced monoclonal antibodies reacted with the surface
of breast cancer cells. however, they had no significant effect on growth of breast cancer
cells in culture medium
Conclusion
Produced monoclonal antibodies against hydatid cyst fluid 40kda band reacted
with the surface of breast cancer cells but had no significant effect on growth of these cells.
