Mhc class i & ii restricted htert derived peptides in cancer immunotherapy

Amir Mohammadi,1 Jamshid gholizadeh navashenaq2,2 Arezoo gowhari shabgah2,3 Reza falak,4 Abasalt hosseinzadeh colagar,5 gholam ali kardar,6,*

1. 1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. 2 Immunology, Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
2. Immunology, Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
3. Immunology, Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
4. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
6. Immunology, Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction

Breast cancer has the highest incidence rate in women in the world. telomerase is the most common tumor associated antigen (taa) and its activity increased in more than 90% of cancers, thus, its targeting can be used as a cancer control

Methods

In this study as a continuing in vitro studies, we re-analyzed htert selected peptides against mice mhc with iedb and epimhc database. about 5 × 105 mouse breast cancer 4t1 cells was washed and suspended in 100 μl normal saline and s.c injected to mammary fat pat of balb/c mice and then start to tumor measurement 2 times a week. about 100 ug klh conjugated peptides accompany incomplete freund′s adjuvant were injected s.c in days 10th and 20th after cancer cells inoculation.

Results

The analysis of peptides shown they are can binding to mice mhc i and ii respectively but score of them is significantly lower than their interaction with human hla

Conclusion

The results were shown contemporary mhc restricted peptide as a vaccine, can inhibit tumor growth and also increase overall survival of vaccinated mice compare untreated group.

Keywords

Htert, mhc, peptide-based vaccine, cancer immunotherapy