oxaliplatinum is one of the drugs used to cancer therapy, but it also has side effects. one of the best sterategy is the use of nano gels, especially chitosan. the aim of this study was cyctoxic effect of chitosan nanogel encapsulated oxaliplatin on colon cancer cell line (ht29) and bax and bcl2 gene expression.
Methods
In this study, cytoxic effect of chitosan nanogel encapsulated oxaliplatin on ht29 cell line was assessed via mtt technique after 24 hours. subsequently, after treatment of ht29 cells with ic50 cocentration of chitosan nanogel encapsulated oxaliplatin, the cell rna was extracted and converted to cdna. finally, bax and bcl2 gene expression comparing to β-actin (refference gene) using real time pcr was analysed.
Results
Treatment of ht29 cells with 100, 50, 25, 12.5, 6.25, 3.125 µg/ml chitosan nanogel encapsulated oxaliplatin showed the 100 µg/ml had more cytoxic effect and it was significant (p<0.001). the ratio of expression of bax band bcl2 genes to reference genes was up-regulated and down-regulated (59/0±3.54,42/0±16/0001).
Conclusion
based on obtaind data, in can be concluded that due to the induction of apoptosis by oxaliplatin, this nanogen can be used in colon cancer treatment.
Keywords
Oxaliplatin, apoptosis, chitosan, real time pcr
oxaliplatin, apoptosis, chitosan, real time pcr
