Hypoxia induced with cobalt chloride could protect mesenchymal stem cells from harsh environment: an in vitro study

Marziyeh Pooladi,1,* Amaneh mohammadi roushandeh,2 Zohreh alizadeh,3 Iraj amiri,4 Mehryar habibi roudkenar,5 Yusef abbasi,6

1. Anatomical Sciences Department, Medicine Faculty, Isfahan University Of Medical Sciences, Isfahan, Iran
2. 1. Anatomical Sciences Department, Medicine Faculty, Gilan University of Medical Sciences, Gilan , Iran
3. 1. Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, Iran
4. 1. Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, Iran
5. 3. Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.
6. 2. Anatomical Sciences Department, Medicine Faculty, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction

Background: using stem cells faced some problems, including low viability and apoptosis after transplantation in the body because of unsuitable conditions like hypoxia, oxidative stresses, and thermal shock. preconditioning of mesenchymal stem cells (mscs) with these can improve their output, although the mechanism is not clear. in this study, the effectiveness of preconditioning with cobalt (ii) chloride on mscs was investigated.

Methods

Materials and methods: mscs were preconditioned with doses of 0, 5, 10, 20, 50, 70, 90, 100, 120, 150 and 200 μm cobalt (ii) chloride for 6,12,24 and 48 hours, then treated with 300 μm for 24 hours. cell viability was evaluated by using the trypan blue test. , tunel test was used to study apoptosis. spss software with one way anova was used for analysis.

Results

results: exposure to cocl2 did not influence cell morphology. we found that preconditioning dose-dependently affected the mscs, as 120 µm after 6, 20µm after 12 and 24hr significantly increased cell viability compared to the control group. the rate of viable cells did not change significantly, compared to the control group (p > 0.05) in longer exposure time. moreover, treatment of 5µm cocl2 significantly decreased apoptosis rate after 48 hours (p < 0.05), although this decrease in apoptosis was not remarkable in other groups.

Conclusion

Preconditioning by cocl2 can improve the cell survival and reduce the rate of apoptosis. the hypoxia could mimic some genes and regulate another involved in cell survival, proliferation, migration and increasing cell antioxidant capacity. therefore preconditioning might provide cellular resistance.

Keywords

Stem cells, cell survival, hypoxia, apoptosis