Evaluation of glutathione reduction (gsh) and total antioxidant capacity (tac) in serum of non-alcoholic and non-diabetic fatty liver

Seyedeh roja Sajadi,1,* Amirhossein esmaeili,2

1. Department of Medical Science, Babol Branch, Islamic Azad University, Babol, Iran
2. Department of Medical Science, Babol Branch, Islamic Azad University, Babol, Iran

Abstract

Introduction

Fatty liver plays a major role in the spread of disease as an oxidative stress factor, inflammation has been reported as a liver damage agent and it plays an important role in the formation of many liver diseases. antioxidant levels of the body by reducing lipid peroxidation and liver necrosis, it can protect the liver to some degree against these injuries. in this study, the level of gsh and tac was evaluated as an antioxidant factor in patients with non-diabetic nafld.

Methods

In this experimental study (case-control), a study was conducted on 85 subjects (50 patients + 35 healthy controls). tac level using the frap method and based on ferric iron reduce to ferrus by calorimetric method, the gsh level was also evaluated by spectrophotometry using the titez method. data analysis was analyzed using spss-21 software, anova was used to analyze the data and tooke and duncan tests were used to compare the means and mean ± sd values were calculated and the difference between the means at the probability level p <0.05 was considered significant.

Results

The results of this study showed that tac levels(μm) in patients with non-alcoholic and non-diabetic fatty liver (1267.0 ± 305.5) compared to healthy control group(967.85 ± 213.2) has significantly increased (p <0.001). while the amount of gsh (μm) in the patient group (275.11± 81.63) compared to healthy group (300.53 ± 10.72) significant decrease was observed (p <0.042).

Conclusion

Since fatty liver induces the production of free radicals ros and rns, it causes lipid peroxidation and oxidative stress in these patients, the increase in tac in these patients is likely to suggest the antioxidant defense system to combat free radicals and oxidative tissue damage. we know that the liver is the main source of glutathione, therefore, liver oxidative damage following fatty liver can be associated with a decrease in serum gsh levels, in addition, glutathione is a direct adjuvant to free radicals and acts as a substrate for the glutathione peroxidase and glutathione s-transferase enzymes to detoxify hydrogen peroxide and also hydroperoxide lipid, therefore, decrease of gsh level in the patient group is more likely than control group.

Keywords

Fatty liver, oxidative stress, total antioxidant capacity (tac), glutathione reduction (gsh).