Determination of sodiuom , lactate and nitric oxide in serum of patient with different degrees of burn

Homeyra Fadaei,1,* Amirhossein esmaeili,2

1. 1-Department of Medical Science, Babol Branch, Islamic Azad University, Babol, Iran
2. 1- Department of Medical Science, Babol Branch, Islamic Azad University, Babol, Iran

Abstract

Introduction

Burn is one of the accidents that threaten about 10 percent of events in the world and causes mortality, physical, mental, economic and disability problems. therefore, our purpose in this study was to examine the relationship between different degrees of burn with serum sodium lactate and nitric oxide levels.

Methods

In this study, 80 samples (50 patient samples +30 control samples) were taken to zare hospital in sari. sodium levels in the samples were analyzed using xd684 electrolyte analyzer, lactate levels using a photometric method and based on the amount of nadh produced by exposure to ldh enzymes as well as nitric oxide levels of the samples using a grease reactioner and is based on chlorometric method . in terms of the results, the data were analyzed using spss_22 software and the mean values of the comparison using the independent t-test program were analyzed and the results were considered significant with p <0.05.

Results

The results of this study showed that the level of sodium (meq / l) in the case group was 136/76± 2/27, compared to the control group, 140/63 ± 3/22, which showed a decrease , (p <0.001) is meaningful too. also, lactate level (mg / dl) in the case group was 21/26 ± 6/38, compared to the control group, 12/17 ± 3/16 this increase was also significant (p <0.001). the results of our research on nitric oxide (μmol/l)in the case group showed a 69/14 ± 28/9 compared to the control group of 43/43 ± 14/93, which also increased with p <0.001.

Conclusion

The results of our study showed that the burn severity with the serum sodium lactate and nitric oxide levels increased the burn percentage with a significant decrease in sodium level (p = 0.009, r2 = 0.1331, y = -0.0389x + 137.97) while increasing the burn percentage with a significant increase in serum level of lactate (p = 0.000 , r2 = 0.6364 , y = 0.2391x + 13.80) and also increased serum nitric oxide levels (p = 0.000, r2 = 0.438 , y = 0.8985x + 41.30).

Keywords

Burn percentage, sodium, lactate, nitric oxide