Investigating the Relationship between Exon 1 Polymorphism of NOEY2 Gene in Women with Polycystic Ovary and Diabetes

Investigating the Relationship between Exon 1 Polymorphism of NOEY2 Gene in Women with Polycystic Ovary and Diabetes
Parichehr Karajibani,¹ Abolhasan Rezaei,^2,*
1. Department of Genetics–School of Basic Science, Islamic Azad University, Tonekabon Branch, IRAN
2. Department of Genetics–School of Basic Science, Islamic Azad University, Tonekabon Branch, IRAN
Introduction: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. Although genetic factors have roles in PCOS, genes that are involved in the etiology of this syndrome are not studied comprehensively. The current study aimed to investigate the relationship between Exon 1 polymorphism of NOEYS2 gene in women with PCOS and diabetes.
Methods: this case-control study was conducted on 240 patients with PCOS and diabetes. Blood sample (1 cc) and genomic DNA of people were extracted and then, the primer was prepared for PCR based on the gene bank. DNA samples approved by Techazma Company were sequenced and the resulted sequences were analyzed by Chromas (version 2.4). Data were analyzed by Assotest method.
Results: polymorphism analysis showed that 5’UTR c.156G>A polymorphism in Exon 1 region of NOEY2 gene exists in people with diabetes, PCOS, and control samples while in people with diabetes-PCOS, no mutation is observed (100% hemology). On the other hand, 5’UTR c.207G+76 G>A polymorphism was observed in Exon 1 region in the control group.
Conclusion: in the investigation of Exon 1 polymorphism of NOEY2 gene, 5’UTR c.156G>A is related to PCOS and diabetes.
Keywords: polymorphism, genetic, NOEY2, PCOS