Evaluating the anti-inflammatory effect of conditioned medium derived endometrial stem cells in endometriosis women

Evaluating the anti-inflammatory effect of conditioned medium derived endometrial stem cells in endometriosis women
Seyedeh Saeideh Sahraei,¹ Ali Kowsari,² Seyedeh Reyhaneh Seyed Ebrahimi,³ Zahra Khodavandpour4,⁴ Faezeh Davoodi Asl,⁵ Azar Sheikholeslami,^6,*
1. 1. Department of Reproductive Biology, the Academic Centre for Education, Culture and Research, Qom Branch, Qom, Iran
3. 3. Department of Anatomical sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4. 4. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
5. 2. Department of Mesenchymal Stem Cells, the Academic Centre for Education, Culture and Research, Qom Branch, Qom, Iran
Introduction: Introduction. Although, the endometriosis is a benign inﬂammatory disorder, it mainly causes complications. Thus, trials for better diagnosis and treatment seems necessary. It is supposed that mesenchymal stem cells derived from menstrual blood (MenSCs) in endometriosis patients are different from that of healthy women and so, they are responsible for the creation of the disease. According to the high expression of inflammatory factors in MenSCs of endometriosis patients, it was supposed that the conditioned medium of these cells from healthy women can affect the expression of inflammatory genes. The aim of this study was evaluating the expression of inflammatory genes in endometriosis patients under treatment of conditioned endometrial from MenSCs of healthy women.
Methods: Methods. Menstrual blood samples (1-2 ml) were collected from endometriosis women and healthy women as control group, in the age range of 22-35 years. Isolation of MenSCs from two groups was performed by the Ficoll-Paque density-gradient method and then endometriosis-derived MenSCs were treated with conditioned medium from control group in passage three. The expression of inflammatory genes including IL-1α, COX-2, TNF-α, NF-kB and HIF-1α were assessed using real time RT-PCR.
Results: Results. Endometriosis-derived MenSCs were morphologically different from healthy MenSCs and also showed higher expression of CD10 marker, which experienced significant decrease in CD10 marker after 4 days’ treatment with conditioned medium of healthy MenSCs. Also, after treatment, Endometriosis-derived MenSCs showed significant decrease in TNF-α and NF-kB expression but no significant decrease in HIF-1α.
Conclusion: Conclusion. Down-regulation of inflammatory genes in endometriosis-derived MenSCs under treatment of conditioned medium derived from healthy MenSCs can be considered as an effective approach for the treatment of endometriosis which needs more investigations.
Keywords: Condition medium, Menstrual blood-derived stem cells, Endometriosis, Inflammatory genes