The effect of conditioned medium derived from endometrial stem cells on expression of stemness genes in endometriosis women

The effect of conditioned medium derived from endometrial stem cells on expression of stemness genes in endometriosis women
Seyedeh Saeideh Sahraei,¹ Ali Kowsari,² Seyedeh Reyhaneh Seyed Ebrahimi,³ Zahra Khodavandpour4,⁴ Naser Kalhor Qom,⁵ Azar Sheikholeslami,^6,*
1. 1. Department of Reproductive Biology, the Academic Centre for Education, Culture and Research, Qom Branch, Qom, Iran
3. 3. Department of Anatomical sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4. 4. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
5. 2. Department of Mesenchymal Stem Cells, the Academic Centre for Education, Culture and Research, Qom Branch, Qom, Iran
6. Department of Mesenchymal Stem Cells, the Academic Centre for Education, Culture and Research, Qom Branch, Qom, Iran
Introduction: Endometriosis described as the presence of endometrial tissues outside the uterine cavity. The etiology of endometriosis is unclear, however, probably retrograde menstruation blood cause this disease. It is supposed that menstrual blood-derived mesenchymal stem cells (MenSCs) in endometriosis patients are different from that of healthy women and so, they are responsible for the creation of the disease. Stemness related genes are upregulated in MenSCs of endometriosis patients, so, it was hypothesized that the conditioned medium of these cells from healthy women can affect the expression of stemness genes. The aim of this study was to evaluate the expression of three genes related to the stemness of endometrial stem cells in endometriosis patients in the presence of endometrial stem cell-derived conditional medium in healthy individuals in vitro.
Methods: Menstrual blood about 1-2 ml was obtained from healthy and endometriosis patients in the age range of 22–35. Endometrial-derived stem cells were isolated by Ficoll-Paque. After cell culture in DMEM containing 10% FBS, treating cells with conditioned medium from control group in passage three was performed. To determine the effect of conditioned medium derived from endometrial stem cells, the expression of stemness markers was evaluated by Real Time-PCR.
Results: In addition to the morphological difference seen in endometriosis-derived MenSCs compared with healthy MenSCs, stemness genes show different expression. The expression of OCT-4 and NANOG were increased in endometriosis-derived MenSCs under treatment for 4 days same as normal MenSCs. Also, SOX2 was showed similar expression after treatment but SALL4 was not showed significant difference.
Conclusion: Recognizing the changes of genes can provide therapeutic strategies for the endometriosis improvement. In addition to, change of expression of stemness markers in endometriosis patients in the presence of conditioned medium derived from endometrial stem cells can be an important strategy for treatment.
Keywords: Menstrual blood-derived stem cells, Endometriosis, Stemness genes, Condition medium