Evaluation of the effect of antibacterial nano-particles containing Trachyspermum Copticum essential oil by Mozaffari method

Evaluation of the effect of antibacterial nano-particles containing Trachyspermum Copticum essential oil by Mozaffari method
Mohammad Javad Forouzani-Moghaddam,^1,* Amir Hossein Kheirkhah,²
1. Msc of Food Microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran
2. Msc of Medical Biotechnology, School of medical, Shahid Sadoghi University of Medical Sciences, Yazd, Iran
Introduction: The aim of this study was to evaluation of the effect of antibacterial of nano-particles containing Trachyspermum Copticum's essential oil. For this purpose, the lipid system containing Trachyspermum Copticum's essential oil has been synthesized for gram-positive (Staphylococcus aureus) and gram-negative (E.coli and Pseudomonas aeruginosa) antibacterial methods as nosocomial infections.
Methods: The type of study is laboratory research. The nanoparticle synthesis method is Mozaffari method. Particle characterization has been performed in terms of size and charge with DLS and morphology with the Atomic Force Microscope (AFM) and the amount of loading and release with the spectrophotometer. MIC and MBC tests were then performed to evaluate the performance of nanoparticles containing Trachyspermum Copticum's essential oil on Staphylococcus aureus, E.coli and Pseudomonas aeruginosa.
Results: The average particle diameter was 70 nm and its zeta potential was -17.8 mV. The loading rate in nanoparticles was 54%, which was calculated by reading the absorption of light from the standard Trachyspermum Copticum curve. The minimum inhibitory concentration (MIC) of Staphylococcus aureus, E.coli and Pseudomonas aeruginosa for nanoparticles was 15.625, 31.25, 31.25 mg / ml. The minimum bactericidal concentration (MBC) of Staphylococcus aureus, E.coli and Pseudomonas aeruginosa for nanoparticles was 31.25, 31.25, 62.5 mg / ml.
Conclusion: Nanoparticles containing Trachyspermum Copticum's essential oil kill Staphylococcus aureus, E.coli and Pseudomonas aeruginosa as nosocomial infection and can be used as antibacterial nanoparticles.
Keywords: Trachyspermum Copticum, Nanoparticle, Antibacterial, Nosocomial infections