Investigating change of AkT gene expression in the T98G Glioblastoma cell line under treatment with thiosemicarbazones complexes (Cu)

Investigating change of AkT gene expression in the T98G Glioblastoma cell line under treatment with thiosemicarbazones complexes (Cu)
Shohreh Shahmir,^1,* Golnaz Asaadi Tehrani,² Sina Mirza Ahmadi,³
1. Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan , Iran.
2. Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan , Iran.
3. Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan , Iran.
Introduction: A brain tumor is an abnormal mass or growth of cells in the brain. There are different types of brain tumors, some of which are benign and some of which are cancerous or malignant. Glioblastoma multiforme GBM is the most common invasive and actually the deadliest type of primary brain tumor in adults. The survival time after initial diagnosis is 12 to 15 months. This cancer has a relatively low prevalence but accounts for 2.5% of cancer deaths. One of the promising sites in which thiosemicarbazone compounds are being developed is their use against cancer. Their antitumor activity is very distinct and highly dependent on the biological type of tumor cells. The purpose of this study was to Evaluation of alternations in Akt gene expression in the T98G Glioblastoma cell line under treatment with thiosemicarbazone complexes (Cu).
Methods: In this study, thiosemicarbazone complexes (Cu) were prepared with 17.5 and 20µM concentrations. The T98G cell line was treated by Cu in 17.5 and 20µM concentrations after cell passage. Then RNA extraction and cDNA synthesis were performed and the expression of the Akt gene and GAPDH gene as the reference gene was investigated by Real-Time PCR, finally, the results were analyzed with Rest software.
Results: Our results confirmed that at both concentrations of 17.5 and 20µM a considerable gene expression decreasing observed after 24 hours of treatment. At 17.5 and 20µM concentrations, expression was detected 0/22 and 0/019 respectively. According to the expression level of the reference gene GPDH 1, the evidence showed a decrease in the expression of the gene under treatment with the thiosemicarbazones complexes Cu.
Conclusion: The results of cell line treatment with thiosemicarbazones complexes Cu of and comparison with the GPDH control gene showed that Cu can be effective in reducing the expression of the Akt gene as an oncogene. The results showed that these changes depended on the concentration of the drug because with increasing the concentration at a constant time, a greater decrease in gene expression was observed.
Keywords: Glioblastoma multiforme, thiosemicarbazones complexes Cu, AKT