- Evaluation of the relationship between PI3K oncogene expression changes in Jurkat E6.1 acute lymphoblastic leukemia cell line treated with NI chemotherapy
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Marjan Kamalpour,1,* Golnaz Asaadi Tehrani,2 Azadeh Mirzaahmadi,3
1. MSc of molecular Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2. Ph.D of molecular Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran
3. Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
- Introduction: Leukemia is characterized by the excessive growth of blood cells that affects the bone marrow. It can also be seen in adults, especially people over 65 years. The aim of this study was to evaluate changes in the expression of AKTPI3K signaling pathway PI3K gene in acute lymphoblastic leukemia cell line Jurkat E6.1 under treatment with NI.
- Methods: NI was prepared at different concentrations (2,5 macro molar).The Jurkat E6.1 cancer cells were treated with chemotherapy after the cell passage in (48 hours). RNA extraction and Cdna synthesis were performed and the PI3K gene expression was evaluated by Real Time PCR. The results were analyzed by REST analaysis softwere.
- Results: The results of this study showed that PI3K gene in concentrations (2 and 5 macro molar) in 48 hours showed a significant decrease (p<0.001) from the expression level of the gene.
- Conclusion: : PI3K gene expression changes are influenced by the NI drug and decrease the expression of this oncogene in all concentrations and time, depending on time and concentration. The results of this study can be used to control and treat the optimal treatment of patients with leukemia, by identifying molecular pathways in the function of the chemotherapy drugs used, as well as introducing new drugs and preventing untreated control of JURKAT cancer cells.
- Keywords: Acute lymphoblastic leukemia, PI3K, NI, Jurkat E6.1