Assessment of different doses of Nickel on BUN, Creatinine and Uric acid values in female Wistar rats

Assessment of different doses of Nickel on BUN, Creatinine and Uric acid values in female Wistar rats
Amirreza Eslami,^1,* Seyed Mohammad Hosseini,² Atena Rahimi,³
1. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
2. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
3. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Introduction: Nickel is known as heavy metal spreading widely over environment. Also, Nickel is presented in many industrial and commercial uses. Although inhalation is considered as one of the main routes of this pollutant transmission, humans in general are exposed to Nickel by contaminated food and water sources. Nevertheless, due to limited absorption of Nickel through gastrointestinal tract, it has less importance compared to other routes. Toxic and carcinogenic effects of Nickel on humans and animals have been investigated via various studies during recent decades.
Methods: 32 female rats were divided equally into 4 groups. The first group considered as control, only received water by intraperitoneal injection and the rest obtained Nickel by doses of 10, 15 and 25 mg⁄kg intraperitoneally. Examination period takes 20 days and on days 8, 12 and 16 rats were injected. On 20th day, blood samples were taken from any rats for evaluating biochemical values and eventually under general anesthesia with Ketamine and Xylazine, rats were euthanized.
Results: Dose-dependent increases occurred in values of BUN, Creatinine and Uric acid. According to the levels of BUN, Creatinine and Uric acid of the 4th group, which are the highest among all the groups, the dose 25 mg⁄kg indicated as the most toxic dose in this investigation.
Conclusion: This study demonstrated that Nickel causes adverse impacts on rats. To sum up, it has been determined that renal toxicity is one of the principal destructive effects of Nickel on bodies.
Keywords: Nickel, Wistar rat, BUN, Creatinine, Uric acid