Biosynthesis of magnetic iron nanoparticles(MINPs) by Lactobacillus fermentum and anti microbial effect of MINPs

Biosynthesis of magnetic iron nanoparticles(MINPs) by Lactobacillus fermentum and anti microbial effect of MINPs
Mina Zolfaghari,^1,* Behin Omidi,² RodabehBehzadi Andohjerdi,³
Introduction: In the last decade, Nanotechnology has made significant development, especially in the medical sciences and used of nanoparticles,researchers has been much attention to synthesis of a different kind of NPs. Among them, Magnetic Nanoparticles are interesting for researchers because they have great usage such as in data storage, catalysis, and bioapplications including magnetic bioseparation and detection of biological entities (cell, protein, nucleic acids, enzyme, bacterials, virus, etc.), clinic diagnosis and therapy such as MRI and MFH , effective drug delivery and biological labels. Several methods used to synthesized magnetic NPs, but biosynthesis is a new way that used plants, algae and microorganism.In this study we used Lactobacillus Fermentum to biosynthesis of magnetic iron nanoparticles
Methods: L.Fermentum)PTCC 1744(was obtained from microbial collection of (IROST).It was inoculated in MRS broth and incubated in 37̊c and 5% co2 for 72 h. then that were centrifuged at 5000 rpm and their supernatant were used for further experiments. Aqueous Fe2O3 solution (10−3 M) was prepared and added to supernatants of L.fermentum(10% v/v) in ambient condition.The production of iron nanoparticles was proved by changing the color of the solution, uv-vis spectroscopy ,XRD ,FTIR and TEM .Antimicrobial effect of them was measured by disc diffusion method.
Results: The color of solution was Indian red, change of color to dark brown showed that INPs were produced.The reduction of the Fe2+ ions by the supernatant of this bacteria and formation of INPs were monitored by UV_visible spectroscopy and they were showed a peak during 400-500 nm.According to the results of FTIR, the effective group in this reaction was the hydroxyl groups (pick in 3331.18 cm-1) and carbonyl groups (pick in 1659.00 cm-1). The formation of INPs was provided by X_ray diffraction (XRD) technique using an X_ray diffractometer with radiation λ = 54.1 Å for Fe3O4 and ƛ=33.2 for Fe2O3 over a wide range of Bragg angles.Based on the documents from the JCPDS data, the diffractions of 200, 311, 511, 440 in the angles 30.4, 35.8, 43.5, 54.1 , show the presence of Fe3O4 magnetic nanoparticles. The presence of a peak at an angle of 33.2 degrees indicates the production of Fe2O3. The shape of them was spherical and dimensions are 34-44 (nm) was expected. TEM results showed thatSize of the INPs formed by the supernatants of Lactobacillus fermentum was 20-30 ±1 nm and spherical shape. The results of the antimicrobial effect was,Inhibitory zone inEscherichia coli19± 0.4mm,Staphylococcus aureus16.5± 0.41mm,Bacillus subtilis39 ±1mm,Pseudomonas aeruginosa11 ±0.5mm ,This results showed that the INPs was effective.
Conclusion: According to results, we have successfully extra cellular synthesized of INPs by Lactobacillus fermentum supernatants. This method is simple, safe, cost effective and eco-friendly.INPs produced by this method are very small and also Lactobacilluses are the major group of probiotics, so this bacteria are safe and without pathogenic effects. So we can definitely used of them in medical and pharmacology purposed
Keywords: Magnetic Iron Nanoparticle , Probiotic , Lactobacillus fermentum ,Biosynthesis