Investigation of the Effect of Phenytoin on the Ovary Differentiation and Expression of the Related Genes in Mouse Embryos

Investigation of the Effect of Phenytoin on the Ovary Differentiation and Expression of the Related Genes in Mouse Embryos
shokripour ramin,¹ zamanzadeh zahra,² ahadi alimohammad,^3,* ranjbar ramesh,⁴
1. Department of Biotechnology, faculty of Biological Sciencen and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
2. Department of Biotechnology, faculty of Biological Sciencen and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
3. Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
4. Department of Biotechnology, faculty of Biological Sciencen and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
Introduction: Epilepsy is a disorder of the central nervous system (neurological disorder) in which the activity of nerve cells in the brain is disrupted and leads to seizures during which abnormal behavior, symptoms and feelings including loss of consciousness, occur. According to the World Health Organization, more than 50 million people worldwide are suffering from epilepsy (1,2,3). There are many drugs that are effective in treating the disease. Phenytoin is one of the most common drugs. The drug enters the cell through the sodium channel to modulate excitatory or inhibitory reactions and acts at different levels of synapses (4,5,6,7).
Methods: In this study, 2 groups of 7 balb / c mice were tested. The treatment group was treated with phenytoin before pregnancy until the birth of the infants and brain tissue sampling was performed. Finally, RNA was extracted from the target tissues, then their cDNA was synthesized and the expression of sox9, rspo1 and lif genes was measured using Real Time-RT PCR technique. Rest 2009 software was used to analyze the expression of genes. The gene network of these three genes was plotted using cytoscape software.
Results: In this study, expression changes according to statistical analysis in rspo1 and lif genes at the level of 0.05 are not significant, but sox9 gene has a 4-fold increase in expression. From the side effects of this drug phenytoin reduced sexual desire and reduced the quality of fertility in treated mice, so that the gestational age of treated mices were increased rather than control mice (About 5 to 7 days after delivery, the control mice gave birth to the treated mice). The number of fetuses born was less than the control group and the eating of rats in the treatment group was very high.
Conclusion: According to this results seems it is better to use an alternative drug that is safe and has fewer side effects than Phenytoin especially during pregnancy and lactation period.
Keywords: Phenytoin, ovary, , LIF, RSPO1, SOX9