Bioinformatics evaluation of targetom has-mir7-1-3p signaling pathway and related function of PCNA, SNP (RS3626) in patients with gastric cancer

Bioinformatics evaluation of targetom has-mir7-1-3p signaling pathway and related function of PCNA, SNP (RS3626) in patients with gastric cancer
arezo nasiran,^1,* massoud hoshmand,² nasrinfattahi,³ atefeh zamani,⁴
1. ashrafi esfahani university
2. medicalgenetic department ,national institute of genetic engineering and biotechnology .teran iran
3. gene raz buali ,genetic and biotechnology academy
4. gene raz buali ,genetic and biotechnology academy
Introduction: gastric cancer remains a major public health issue as the fourth most common cancer and the second leading cause of cancer death worldwide Environmental factors as well as genetic factors play an important role in the development and progression of this disease. With the introduction of microRNA into the genetic domain, these powerful molecules are opening up their place in the genetic domain of disease. miRNAs are small, non-coding molecules involved in various cellular processes, such as cell differentiation and death. Using bioinformatics databases PCNA gene with SNP (rs626) is effective in cell cycle signaling pathways in gastric cancer.
Methods: different genetics and environment risk factors can cause this csncer . therefor for fainding more bioinformatic informathion we use miRsnp,NCBI, miRbase,miRWAIK2.0,DAVID database and KEGG pathway
Results: in this study , bioinformatics predicted that , this microRNA (has-mir7-1-3p) is expected to target the rs3626 in coding region of the PCNA
Conclusion: the expected expression of PCNA and the negative regulatory function of the microRNAs would be expected . hsa-miR-7-1-3p increase and then decrease the expression of its target gene . for this reason ,the hsa-miR-7-1-3p is predicted to have a higher binding capacity to the rs3626 mutant(C) allele than to the dominant (G) allele of this SNP and may therefore be a risky mutant allele and cause cancer
Keywords: Gastric cancer, Single nucleotide polymorphism, ra3626, hsa-miR-7-1-3p , PCNA