Controlled Delivery of Brain Derived Neurotrophic Factor and GoldNanoparticles from Chitosan/TPP Nanoparticles for Tissue Engineering Applications

Controlled Delivery of Brain Derived Neurotrophic Factor and GoldNanoparticles from Chitosan/TPP Nanoparticles for Tissue Engineering Applications
Reihaneh Seyedebrahim,^1,* Shahnaz Razavi,² Jaleh Varshosaz,³
1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Brain derived neurotrophic factor (BDNF) is highly effective in the treatment of various diseases, and selected for growth factor delivery system. Also, gold-nanoparticles (AuNPs) plays the paramount role in diagnosis and treatment of diseases.
Methods: The aim of this study is to encapsulate BDNF and AuNPs in chitosan nanoparticles and its effect on human adipose derived stem cells (h-ADSCs) proliferation was evaluated. The ratio of 1:3 chitosan/TPP was determined as the optimum ratio, BDNF or AuNPs were added to 0.1% chitosan solution for preparation of nanoparticles. The release rate of BDNF and AuNPs were assessed by Bradford test and inductive coupled plasma optical emission spectrometry technique, respectively.
Results: In this study, BDNF and AuNPs loading efficiency were 89.46 ± 2.77 (%) and 93.8 ± 2.12 (%), respectively. The kinetic release of BDNF is 83.28 ± 2.22 (%) and AuNPs release rate was 48.4 ± 0.108(%), during the 7 days. MTT findings demonstrated h-ADSCs viability in the presence encapsulated BDNF and AuNPs is significantly more than control group on 3 and 7 days
Conclusion: These findings indicate the controlled release of encapsulated BDNF and AuNPs, and synergist effects of nanoparticles on h-ADSCs viability; it suggests an efficient delivery of BDNF and AuNPs, for tissue engineering.
Keywords: BDNF , Chitosan, Delivery system, Gold nanoparticle, Controlled release