Evalution the efficacy of hydroalchoholic extraction of Verbascum Thapsus.L leaves in rat burn wound model

Evalution the efficacy of hydroalchoholic extraction of Verbascum Thapsus.L leaves in rat burn wound model
Sepideh zarei,¹ Seyed Zahra Mousavi,^2,*
1. Sepideh Zarei , Pharm.D student, Faculty of Pharmacy and Pharmaceutical Sciences , Tehran Medical Sciences, Islamic Azad University,Tehran-Iran (IAUPS)
2. Seyed Zahra Mousavi, Pharm D., Ph.D. Associate Professor of Toxicology, Department of Pharmacology-Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences , Tehran Medical Sciences, Islamic Azad University,Tehran-Iran (IAUPS)
Introduction: Wound healing has always been a major concern for physicians and dermatologists, and burn healing is still a challenge in modern medicine. Burn wounds are one of the main causes of superficial scars on the body, which may lead to abnormalities such as isolation or depression regardless of the problem of treatment and cost. Verbascum thapsus.L is a plant of the scorphulariacea family. This plant has several therapeutic uses in traditional medicine, including the treatment of burn wounds. Also, according to previous studies, its antibacterial and healing effects have been proven. In this article, the efficacy of topical application of hydroalcoholic extract of Verbascum leaves in the second-degree burn wound model in male rats is evaluated.
Methods: After dentification and preparation of hydroalcoholic extract of Verbascum leaves by using percolation method, a topical formulation was made by inserting the extract into the eucerine base.Fallowing burn wounds induction, 30 male wistar rats were randomly divided into 5 groups: Sham positive control " silver sulfosodiazine ointment", negative control "Eucerine" as ointment base, and two groups 2.5% and 5% w/w of plant extract formulation . All groups received specific medication every 24 hours and dressing was changed every day.By photographing the wound surface over a period of 21 days, the percentage of wound healling and contraction were examined. On the last day of examination, all rats sacrificed and process of wound tissue healing and histological changes were evaluated.
Results: Macroscopic examination of the wound surface showed a significant size reduction in burn wounds of rats receiving 2.5% and 5% extract, which was more noticeable in the 5% extract group. In addition, based on histopathological evaluation, fewer inflammatory cells were observed in the wound tissue of rats treated with the extract formulations compared to 3 other groups. Also, epithelialization and increase in the number of new capillaries in the two groups receiving 2.5% and 5% extract were significantly higher than the other groups and the wound tissue in the extract group was almost completely healed.
Conclusion: Topical formulation containing hydroalcoholic extract of Verbascum leaves can be used as an herbal product to heal burn wounds.
Keywords: Verbascum thapsu , Mullen , rat , burn wound , wound healing