Evaluation of the effects of a new drug derived from the combination of ninhydrin with Mafenide on Pseudomonas aeruginosa

Evaluation of the effects of a new drug derived from the combination of ninhydrin with Mafenide on Pseudomonas aeruginosa
Mahdieh Ramezani,¹ Sorour Aalipanah,^2,* Shohreh Nasiri poroj,³ Koroush Motaghinia,⁴ Parisa Khiabanirad,⁵
1. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
4. Department of chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
5. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen. This organism is resistant to different groups of antibiotics. Infections caused by this organism due to antibiotic resistance may eventually lead to death. It is a gram-negative, aerobic, oxidase-positive and motile bacteria. Mafenide is a sulfonamide that has a bacteriostatic effect against many gram-negative and positive bacteria such as Pseudomonas aeruginosa and specified strains of anaerobes. It is absorbed through areas without blood vessels and is rapidly metabolized and excreted by the kidneys. Ninhydrin (Dihydroxy Indane-13-dione-22) is a chemical used to identify type I amines and type II amines. A chemical synthesis begins with the selection of compounds known as reactants. Various types of chemical reactions can be performed on these compounds to synthesize a particular product. In order to obtain new pharmaceutical compounds and increase antibacterial properties, new derivatives of Mafenide were synthesized.
Methods: 0.1 mmol of Mafenide acetate and 0.1 mmol of ninhydrin in 10 ml of ethanol was reflux for 3 hours. The reaction progress was controlled using thin-layer chromatography (TLC) in a normal hexane-ethyl acetate tank in a ratio of (1: 4). The solvent was evaporated, the precipitate was crystallized with 2-propanol solvent. Finally, the structure of the synthesized compound was confirmed by determining the melting point, C-NMR, H-NMR, TLC and IR. Then the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the synthesized drug on Pseudomonas aeruginosa were determined exerting well method on Müller-Hinton agar medium.
Results: Different colour and melting point and the results of C-NMR, H-NMR, TLC and IR indicate the new structure of the synthesized drug. The MIC and MBC of Mafenide on Pseudomonas aeruginosa were 0.5 and 1 mg/ml, and for the new derivative of Mafenide were 0.3 and 0.5 mg/ml.
Conclusion: According to the results, the synthesized drug has a more effective antibacterial activity against Pseudomonas aeruginosa than the base drug which can be used as an ointment in burn patients and people with bedsores after secondary evaluation.
Keywords: Mafenide Acetate, Reflux method, Pseudomonas aeruginosa, Drug resistance, Antimicrobial effect