Evaluation of trace elements and blood biochemical parameters in diabetic rats supplemented with Spirulina platensis microalgae: Investigation of mechanism by antioxidant enzymes in liver

Evaluation of trace elements and blood biochemical parameters in diabetic rats supplemented with Spirulina platensis microalgae: Investigation of mechanism by antioxidant enzymes in liver
Morteza Ghasemi,¹ Linda Soeibipour,² Nanuli Doreulee,³ Nasrollah moradi-kor,^4,*
1. Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
2. Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia
3. Professor of Biological Sciences, Faculty of Exact and Natural Sciences, Head of direction of Human and Animal Physiology, Tbilisi State University, Tbilisi, Georgia.
4. Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia
Introduction: Objectives: Lipid peroxidation and hyperglycemic are known as common signs for diabetes. On the other hand, antioxidants, such as Spirulina platensis microalgae (SPM), may prevent lipid peroxidation and hyperglycemic. Thus, this study aimed to evaluation the antioxidant mechanism of SPM on lipid peroxidation and hyperglycemic in diabetes rats.
Methods: Methods: Sixty-four rats were grouped to 8 groups (n=8) and orally treated with 0, 10, 20, 30 mg.kg-1 body weight of SPM extract. Experimental groups were as follows; diabetic rats fed with 0 mg.kg-1SPM (DC), 10 mg.kg-1 SPM, 20 mg.kg-1SPM and 30 mg.kg-1 of SPM, respectively. Healthy rats were treated with 0 mg.kg-1 SPM (HC), 10 mg.kg-1 of SPM, 20 mg.kg-1 of SPM and 30 mg.kg-1 SPM. At the end of trial, blood samples were collected and the plasma concentrations of trace minerals (TMs), biochemical parameters, antioxidant enzymes in liver were assessed.
Results: Results: Our findings showed that diabetes significantly lowered the plasma concentration of TMs and antioxidant enzymes in liver and also increased the levels of malondialdehyde, glucose and lipid profile (DC vs. HC), but oral supplementing with SPM, (20 and 30 mg.kg-1 body weight), lowered levels of malondialdehyde level, glucose and lipid parameters. The same levels increased the plasma contents of zinc, iron, copper and selenium and activity of antioxidant enzymes (P<0.05).
Conclusion: Conclusion: It can be concluded that diabetes lowered TMs concentration and antioxidant enzymes and also increased lipid profile and glucose concentration, but SPM supplementing (20 and 30 mg.kg-1 body weight) increased some TMs and antioxidant enzymes. SPM may provide TMs for synthesis of antioxidant enzymes which subsequently enzymes reduce lipid profile and glucose concentration.
Keywords: Keywords: Antioxidant enzymes, Diabetes, Lipid profile, Plasma selenium, Spirulina platensis