PREPARATION AND CHARACTERIZATION OF HIGHLY POROUS NANOCLAYENRICHED POLY (Ɛ-CAPROLACTONE) SCAFFOLDS AND EVALUATION OF OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS

PREPARATION AND CHARACTERIZATION OF HIGHLY POROUS NANOCLAYENRICHED POLY (Ɛ-CAPROLACTONE) SCAFFOLDS AND EVALUATION OF OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS
seyedeh elnaz enderami,^1,* seyedeh sara shafiei,² mehdi shamsara,³ seyed ehsan enderami,⁴
1. Stem Cell and Regenerative Medicine,Institue of Medical Biotechnology, National Institue of Genetic Engineering & Biotechnology, Tehran, Iran
2. Stem Cell and Regenerative Medicine,Institue of Medical Biotechnology, National Institue of Genetic Engineering & Biotechnology, Tehran, Iran
3. National Research center for Transgenic Mouse, National Institue of Genetic Engineering & Biotechnology, Tehran, Iran
4. Immunogenetics research center, Department of Medical Biotechnology, Faculty of Medicine, Mazandaran university of Medical Sciences, Sari, Iran
Introduction: Introduction: In the last decades, tissue engineering has an effective role in introducing osteo Bioimplants1.Co-cell polymer with nano-clay can be one of the best implants for taking the place of bone failing and defecting in patients2,3. Human mesenchymal stem cells (hMSCs) and nano clay polymeric scaffolds can be biocompatible and biodegradable in the body4. The nano clay_enriched polycaprolactone (PCL) scaffolds aid hMSCs proliferation and adhesion 5. Layered double hydroxide (LDH) nano clay can improve PCL properties in stem cellsdifferentiation3. Therefore the aim of this study is to fabricate bio-active and bio-interactive highly porous(more than 94%) PCL scaffolds composited with different amounts of LDH at low temperatures.
Methods: In this study, highly porous PCL scaffold doped with different contents of LDH (ranging from 0.1 wt.% to 10 wt.%) simultaneously and the LDH attended smoothly dispersed throughout the PCL scaffold as confirmed by energy dispersive X_ray analysis (EDX). The viability, proliferation, morphology, attachment, and osteogenic differentiation of hMScs were assessed, and also mechanical behavior, degradation, and porosity of scaffold were examined. The osteo genes expression did for confirmation osteogenic differentiation of hMSCs.
Results: This nanocomposite scaffold containing 1wt% of LDH_enriched PCL showed more excellent mechanical properties compared to that pure PCL. The presence of LDH in polymeric scaffold promoted in vitro bio-mineralization and osteogenic differentiation. Experimental tests demonstrated that highly porous scaffold has no toxicity on the cells and also causes cell adhesion and proliferation.
Conclusion: These results demonstrated that the addition of LDH improved the osteogenic differentiation while compression strength, hydrophilicity, and degradation rate were also increased. Overall this nanocomposite scaffold could be a suitable candidate for bone tissue engineering scaffold applications.
Keywords: Keywords: layered double hydroxide, polycaprolactone, nano clay, bone tissue engineering