Evaluation of changes in hepatic tissue , enzymes and inflammation cytokines due to experimental common bile duct obstruction in rat model

Evaluation of changes in hepatic tissue , enzymes and inflammation cytokines due to experimental common bile duct obstruction in rat model
Ehsan Soleimaninejadian,¹ marziyeh sadeghi,^2,* Moosa Javdani,³ Abdonaser Mohebi,⁴
1. department of biology, faculty of Science, Shahrekord University, Shahrekord, Iran
2. shahrekord univercity
3. shahrekord univercity
4. shahrekord univercity
Introduction: There is controversy among clinicians for diagnostic bile duct obstruction. The primary aim of this study was to examine the histopathological changes of the liver and the level of inflammatory factors (TNF-α), Tumor necrosis Factor-α, and Interleukin-6 (IL-6) during the obstruction. The secondary goal of this study was to examine the liver enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bile acids (TBA), and gamma-glutamyl transferase (GGT).
Methods: Consequently, we purchased thirty-five rats. The rats were housed for two weeks to adapt to the new conditions. We randomly placed these animals into three groups. The first control group (control-1) with five rats, the second control group (control-2) with fifteen rats, and the case group with fifteen rats. Both control-2 and case groups received ketamine, xylazine, and isoflurane to induce anesthesia. Although, abdomens of the rats in control-2 were knifed, but the bile duct obstruction was not induced. In spite, the abdomens in the case group lacerate and portal layers of the main bile ducts were chocked by using silk sutures. Hepatic tissue and serum of the rats in all the three groups were analyzed every two days. Hematoxylin/Eosin followed by microscopic observation were applied to study the liver tissues. Also, the biochemical analysis of the serum sample was done using an auto-analyzer.
Results: Our results revealed a steady increase in levels of TBA, TNF-α, and IL-6 during the bile duct clog. In spite, we could not see any significant changes in the liver enzymes such as AST, GGT, and ALT in the case group, in comparison to both control groups. Histopathology tests reveal inflammatory cells around the portal area in liver tissues. Besides, necrosis and ballooning have been seen around the portal area
Conclusion: . Thus, rising the liver enzyme such as TBA in addition to inflammatory factors such as TNF-α and IL-6 can be employed to diagnose the biliary duct obstruction in the first weeks
Keywords: Key words: Bile duct obstruction, Bile duct inflammation, Liver enzymes, Steatosis, TNF-α, IL-6