Study of the Electrospun Nanocomposite Scaffold effect on propagation of Human Spermatogonial Stem Cells

Study of the Electrospun Nanocomposite Scaffold effect on propagation of Human Spermatogonial Stem Cells
Maria Zahiri,^1,* Hamed Allahyari,² Banafshe Esmaeilzade,³ Mohammadali Zare,⁴
1. 1. The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran 2. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
2. Student Research Committee, Bushehr University Of Medical Sciences, Bushehr, Iran
3. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
4. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
Introduction: The process of spermatogenesis in humans is complex and unknown, and spermatogonia stem cells are important because of their role in transmitting hereditary information. Efforts to provide a body-like environment for various research and therapeutic purposes have always been considered. In this study, the culture system on the electrospun polycaprolactone / gelatin scaffold was evaluated for its efficiency in the proliferation of human spermatogonial stem cells.
Methods: For this purpose, nanocomposite porous scaffolds were prepared using electrospinning method and their structure was confirmed using scanning electron microscope (SEM) and Characterization methods. Isolated human spermatogonial stem cells (SSCs) were cultured on designed scaffolds and their viability and proliferation during two weeks of culture were compared with the control group.
Results: SEM studies showed that the resulting porous scaffold had suitable diameter and arrangement. The scaffold was biocompatible and biodegradable. Also, Studies on cell activity and proliferation showed a significant increase in the number of spermatogonia stem cells in the scaffold culture group compared with the control (P 0.05). Immunocytochemical findings confirmed the presence of human spermatogonia stem cell colonies in cultural groups.
Conclusion: Overall, the resulting electrospun scaffold seems to provide a suitable capacity for self-regeneration of human spermatogonia stem cells and could have a good application potential for use in research and clinical regenerative medicine of male infertility.
Keywords: Spermatogonia stem cells, polycaprolactone, gelatin, electrospinning