Comparison of the effect of Brassica oleracea and letrozole on aromatase inhibitor enzyme and steroid hormone levels and oocyte development in Trichogaster trichopeterus

Comparison of the effect of Brassica oleracea and letrozole on aromatase inhibitor enzyme and steroid hormone levels and oocyte development in Trichogaster trichopeterus
Tahereh Naji,^1,* Homayoun Hosseinzadeh,² Zohreh Kamalizadeh,³ Ziba Kolouei,⁴
1. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2. Iranian Fisheries Science Research Institute, Agricultural Research and Education Organization, Tehran, Iran
4. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Introduction: Today, traditional medicine and the use of herbal extract have found a special place among the patients. The abundance and the ease of availability of the Brassica oleracea has made it popular in some countries. Recent analyzes on the compounds found in Brassica Oleracea has shown a magnificent effect on the ovary, pituitary-gonadal axis and aromatase inhibitor. Therefore, further researches can be done in order to use this plant instead of Letrozole (as a chemical drug) in the treatment of metastatic breast cancer in postmenopausal [1,2,3].
Methods: In this research,120 female fish speciments of Trichogaster trichopterus with average weight of (3-4) grams in 9 treatments were divided into control groups,control1 (intact) and control2 (ethanol injection) and control3 (normal saline injection) and treatment groups receiving 200, 400, 800mg / kg alcoholic extract of Brassica oleracea and 0.1, 0.5 ,1 mg /kg of letrozole. All the prescriptions were administered 10 times in 3 replicates one day in between in intramuscular (IM) injection within 20 days. At the end of the experiments, the fish were anesthetized with clove extract , ovarian histology and sex hormones and liver enzyme were studied in treatments and were compared with control groups.
Results: The statistical results of the level of sex hormones liver enzymes showed significant differences between in the controls groups and treatments (P‹0.05). Also the results of this research showed the inhibitory effect of Brassica oleracea extract and letrozole on oocyte maturation in mature female three spot Gourami.
Conclusion: The result of this study showed that extract of Brassica oleracea such as letrozole can inhibits aromatase enzyme and barrier of oocyte maturation in female three spot Gourami. letrozole can increases the liver enzymes and changes in fat cells but Brassica oleracea decreases the liver enzymes.
Keywords: Brassica oleracea, letrozol, Trichogaster trichopterus, ovary, liver