Evaluation of nano-lipid system containing curcumin extract against E.coli and S. aureus by Mozaffari method

Evaluation of nano-lipid system containing curcumin extract against E.coli and S. aureus by Mozaffari method
Mohammad Javad Forouzani-Moghaddam,^1,*
1. Msc of Food Microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran
Introduction: The aim of this study was to characterize the nano-lipid system containing curcumin extract against E.coli and S.aureus. For this purpose, a lipid system containing curcumin extract has been developed by Muzaffari method.
Methods: The type of study is laboratory research. This nanoparticle is made by Mozaffari method. Particle characterization has been performed in terms of size and charge with DLS and morphology with the Atomic Energy Microscope (AFM) and the amount of loading and release with the spectrophotometer. MIC and MBC tests were then studied to evaluate the performance of nanoparticles containing curcumin extract on E.coli and S.aureus.
Results: The average particle diameter was about 57 nm and its zeta potential was -15.6 mV. The loading rate in nanoparticles was 92.6%, which was calculated by reading the absorption of light from the standard curcumin curve. In addition, minimum inhibitory concentration (MIC) of nanoparticles of E.coli and S.aureus was 15.625 and 31.25 mg / ml and minimum bactericidal concentration (MBC) of E.coli and S.aureus was 31.25 and 31.25 mg / ml.
Conclusion: nano-lipid system containing curcumin extract kill gram-positive (Staphylococcus aureus) and gram-negative bacteria (E.coli) and can be used as antibacterial nano-systems.
Keywords: E.coli, Staphylococcus aureus, Curcumin, Nano particle.