Antibacterial activity of nickel and nickel hydroxide nanoparticles against multidrug resistance E. coli isolated urinary tract

Antibacterial activity of nickel and nickel hydroxide nanoparticles against multidrug resistance E. coli isolated urinary tract
Marzieh ebrahim pour,^1,* Mohammad Javad Forouzani-Moghaddam,²
1. Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2. Msc of Food Microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran
Introduction: Antibacterial materials are so significant in the textile industry, water disinfection, medicine, and food packaging. Unfortunately, organic compounds for sterilization show toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing.
Methods: Nickel and nickel hydroxide nanoparticles (NiNPs and Ni(OH)2-NPs) were prepared and characterized by DLS, SEM, AFM and ATR. Antibacterial activity assay was carried by Spot on lawn method against two selected standard pathogenic bacteria such as E. coli (as Gram negative), S. aureus (as Gram positive) and multidrug resistance E. coli. Also the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against two selected standard pathogenic bacteria and multidrug resistance E. coli.
Results: The formation of the NiNPs and Ni(OH)2-NPs were confirmed by DLS, SEM, AFM and ATR. Antibacterial activity of nanoparticles were confirmed against two selected standard pathogenic bacteria such as E. coli and S. aureus. And also, NiNPs and Ni(OH)2-NPs revealed fair antibacterial effect against multidrug resistance E. coli based on MIC and MBC data. As well, the experimental data presented that the antibacterial activity of NiNPs was more than Ni(OH)2-NPs.
Conclusion: Based on the achieved results, NiNPs and Ni(OH)2-NPs show antibacterial activity against clinical patients bacteria (multidrug resistance E. coli(. Finally, the NPs evaluated in this study have promising properties for applications as antiseptic agent for environment; however, further studies are warranted such as study toxicity NPs on normal human cell line and other clinical bacteria.
Keywords: Antibacteial, NiNPs, Ni(OH)2, Nanoparticles, Pathogen