Increasing fertility IUI procedure in ischemia/Reperfusion treated with hydroalcoholic clove extract powder in adult female mice

Increasing fertility IUI procedure in ischemia/Reperfusion treated with hydroalcoholic clove extract powder in adult female mice
Marzieh Hadavy,^1,* Hengameh Hooshiar Chaman Ara,² Jina khayatzadeh,³ Soroosh Montazeri,⁴ Zakiyeh Salahi,⁵ Maryam Moghimian,⁶
1. Biology Dept.,Science Faculty,Mashhad Braneh,Islamic Azad university, Mashhad,Iran.
2. Biology Dept.,Science Faculty,Mashhad Braneh,Islamic Azad university, Mashhad,Iran.
3. Biology Dept.,Science Faculty,Mashhad Braneh,Islamic Azad university, Mashhad,Iran.
5. Biology Dept.,Science Faculty,Mashhad Braneh,Islamic Azad university, Mashhad,Iran.
Introduction: Considering the significance of reproduction in animal survival , finding a way to eliminate of sterility among women in Torsion or Ovarian Torsion ,of which appropriate untreatment by interruoting perfusion to ovarian will cause to destroy it.Using herbs has been the basis of traditional medicine , and nowadays these plants are the structural basis of chemical drugs, too. Carnation has always been attracted among various herbs and spices,because of its abtioxidant properly and likely it can be a suitable choice to improve or treat sterility induced by ovarian torsion.On the other hand,numerous clinical and experimental treatments for sterility are in progress,one of which is IUI. This method is cost-effective and less invasive than IVF .
Methods: In the present study ,protective effact of incubated hydroalcoholic extract of carnation on torsioned ovarian in rats and also on sterility treatment induced by Ischemia/Reperfusion following IUI method, was studid. For this purpose,20 rats were randomlyd divided into 4 groups. After implementing IUI ,the number of pregnant rats by method was counted:Sham group as control group,group2 :rats under torsion which did not receive treatment with hydroalcoholic carnation extract,group 3:rats which were torshioed and treated by 30 mg or carnation,group 4: rats which were torshioned and treated by 60 mg of carnation extract.
Results: The results indicated that use of this plant is effective on increasing ovarian follcles of female rats and the number of offspring thtough follicle.
Conclusion: It seems that incubated hydroalcoholic oxtract of carnation plays a role in treating sterility, hence, it is suggested for supplementary studies of animal and human.
Keywords: Ovarian Torsion, Rats, Ischemia/Reperfusion, IUI