Angiogenesis inhibition improves asthmatic attacks

Angiogenesis inhibition improves asthmatic attacks
Seyed Mohammadreza Bolandi,¹ Zohreh Abdolmaleki,^2,* Mohammad-Ali Assarehzadegan,³ Mohammad kiani,⁴ Ali Allahgholipour,⁵ Soroush Tohidi,⁶
1. Department of pharmacology, Faculty of veterinary medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
2. Department of pharmacology, Faculty of veterinary medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
3. Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
4. Department of pharmacology, Faculty of veterinary medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
5. Department of Clinical Science, Faculty of veterinary medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
6. Department of Clinical Science, Faculty of veterinary medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
Introduction: Studies on the bronchial vascular bed revealed that an amount of blood vessels in lamina propria and submucosa of the lung tissue in patients who suffered from mild and severe bronchial asthma. So, in this study, a new strategy was used in respiratory system disorders by angiogenesis inhibition in an ovalbumin-induced rat model of asthma.
Methods: Twenty one male Wistar albino rats, 8 weeks old, weighing between 250 and 300 gr were randomly divided into three groups (n= 7 in each) including (1) Control group, (2) OVA-treated group and (3) OVA + Avastin (Avastin drug). On days 1 and 8, 1 mg of OVA and 200 g of aluminum hydroxide in 0.5 mL sterile phosphate-buffered saline (PBS) were intraperitoneally injected to rats in groups 2 and 3. The control group were only subject to intraperitoneal injection of saline on day 1 and day 7. One week after the last injection, the rats (groups 2 and 3) were exposed to OVA inhalation for 30 min at 2-day intervals from days 15 to 25. After preparation of asthma rat models through OVA exposure protocols, the OVA+Avastin group (group 3) were treated with5 mg/kg Avastin drug.
Results: Genes and proteins expression of cytokines in lung tissue and the expression of VEGF protein were assessed in lung tissue. Ovalbumin exposure increased mucosal secretion and VEGF expression and expression of cytokine factors (p≤0.05). However, rats in OVA+Avastin group showed significantly decrease in VEGF and IL-1β and TNF-α genes and proteins (p≤0.05).
Conclusion: The results showed that Avastin efficiently eliminated bronchial inflammation via down regulation of VEGF expression, followed by inflammatory cytokines reducing.
Keywords: Avastin; Asthmatic attacks; IL-1β; TNF-α