Investigation of Chemical compounds and Antibacterial Activity of Capsicum Annum essential oil on Candida albicans and Pseudomonas aeruginosa Invitro

Investigation of Chemical compounds and Antibacterial Activity of Capsicum Annum essential oil on Candida albicans and Pseudomonas aeruginosa Invitro
Neda Movahedi saber,^1,* Gholamreza Bakhshi,² Nasehi Behzad ,³ Alizadeh ,Behroz,⁴
1. M.Sc. Student of Biotechnology, Department of Sciences , Payame Noor University (PNU), Iran
2. Department of Sciences, Payame Noor University (PNU), Iran
3. Department of Agricultural Engineering and Technology; Payame Noor University (PNU), Iran
4. Department of Food Science and Technology, Faculty of Animal Science and Food Technology
Introduction: In recent years Bacterial resistance to antimicrobial agents, is one of the important problems in Nosocomial infections. Capsicum Annum is highly valued for its therapeutic properties. The aim of this study was to identify the evaluation of Capsicum Annum essential oil antibacterial activity on Pseudomonas aeruginosa and Candida albicans in vitro.
Methods: In this paper,the Capsicum Annum essential oil,was extracted using Disk diffusion method was used to measure the diameter of the inhibition zone, and broth microdilution method was used to determine the minimum inhibitory concentration(MIC) and The components of the essential oil,were identified by gas chromatography/mass spectrometry(GC/MS)device.The minimum bactericidal concentration (MBC), was determined using tubes or wells, where no color hange was observed.
Results: The results showed that, 13 compounds, were identified in Capsicum Annum essential oil. The major compound of the essential oil was 2,3-dihydroxypropyl elaidate, 42.78% and oleic acid, 62.20% (42.63% of the total compounds). The diameter of inhibition zone for Pseudomonas aeruginosa ,Candida albicans were obtained 15, and 16 mm, respectively. MBC of Capsicum Annum essential oil for Pseudomonas aeruginosa and for Candida albicans, was 512mg/ml, respectively. However, increasing the concentration of Capsicum Annum essential oil reduced the diameter of the inhibition zone oncentration (512 mg / ml), prevented the growth of this yeast.
Conclusion: According to the results of this study, the Capsicum Annum essential oil has antibacterial effect which is higher in comparison with antibacterial effect of Tetracycline .The comparative results of the antimicrobial effect at concentrations of 1000 and 500mg / ml used of Capsicum Annum essential oil with tetracycline showed that 41/5% essential oil is more effective.On the other the maximum inhibitory diameter of Candida albicans yeast is lower at the highest concentration than the antibiotic amphotericin B.Thereforer Capsicum Annum essential oil can be presented as a new antimicrobial compound of the essential oil.
Keywords: Capsicum Annum ; Chemical composition; Antibacterial ;Essential Oils.