A comparison betweenTaraxacum pregrinium anticancer activity and Cisplatin against human MCF7 and Hek293 cancer cell lines

A comparison betweenTaraxacum pregrinium anticancer activity and Cisplatin against human MCF7 and Hek293 cancer cell lines
Alireza Akhzari,¹ Hoda Abolhasani,^2,*
1. Student Research Committee, Qom University of Medical Science, Qom, Iran
2. Cellular and molecular research center, Qom University of medical Sciences, Qom, Iran
Introduction: Cancer is a chronic and non-communicable disease with genetic and acquired background. Breast cancer is the most common cancer in women and the leading cause of death. The dandelion plant with the scientific name of Taraxacum pregrinium is one of the plants that has a wide growth all over the world and its anti-inflammatory, anti-tumor, apoptotic, antioxidant and immune system stimulation effects have been proven in various researches. In Iran, Taraxacum Peregrinum species of this plant is found in abundance. Therefore, it is important to study the pharmacological effects of dandelion for further research and development on a variety of diseases, including cancer.
Methods: In this study, ethanolic extract of Taraxacum pregrinum root using 96% ethanol solvent was obtained by soaking method with a ratio of 1 to 20. Then 6 dilutions (0.01, 0.1, 1, 10, 100, 1000 and μM) of ethanolic extract of dandelion root and also 6 dilutions (0.01 to 1000 μM) of cisplatin (as one of the drugs of choice for breast cancer treatment) were prepared. The cytotoxic effects of Taraxacum pregrinum ethanolic extract and cisplatin anticancer drug were evaluated using MTT assay on MCF-7 cancer cell line (human breast cancer) and Hek293 healthy cell line (human embryo kidney). Finally, light absorption at 570 nm was read by ELISA reader and data analysis was performed by Exell software and GraphPad Prism 6.
Results: Ethanolic extract of taraxacum pregrinum root ethanolic extract was obtained during 72 hours of exposure but was not significantly effective compared to cisplatin used as a breast cancer drug in this study. But human Hek293 was not toxic to the healthy cell line either.
Conclusion: According to the results of this study, the active ingredients of the taraxacum pregrunium exhibit cytotoxic effects in different human cancer cell lines so it can be applied as Assistance treatment agent in Mcf7 cancer treatment filed.It may be possible to obtain better cytotoxic effects from this plant by changing the extraction method and solvent, the type of cancer cell line, and changing the concentration of the extract.
Keywords: MTT assay-Taraxacum pregrenium-cisplatin-Hek293-MCF7