مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Comparing the Effects of Methanol Extract of Ficcus caricaand Spirulina platensis with Silybum marianum on Liver enzymes and Hematological factors in the Model of Fatty Liver Induced by Carbon Tetrachloride of Cyprinus carpio
Comparing the Effects of Methanol Extract of Ficcus caricaand Spirulina platensis with Silybum marianum on Liver enzymes and Hematological factors in the Model of Fatty Liver Induced by Carbon Tetrachloride of Cyprinus carpio
Tahereh Naji,1,*Homayoun Hosseinzadeh,2Shahrbanoo Nobakht Sarokalaei,3Ziba Kolouei,4
1. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. 2. Iranian Fisheries Science Research Institute, Agricultural Research and Education Organization, Tehran, Iran 3. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. 4. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Introduction: Fatty liver in medicine is a reversible condition of the accumulation of fat vacuoles in the liver cells, which is characterized by liver inflammation. The main causes of fatty liver in Iran are diabetes, obesity, malnutrition and sudden weight loss. Defects in blood factors can cause many problems, including weakness, lack of concentration, hot flashes, increased risk of infection, shortness of breath, depression, insomnia, and decreased sex. Fatty liver disease is diagnosed by elevated liver enzymes.
Methods: In this study, 100 male and female Cyprinus carpio, with 15±5 gr weight, were divided into 10 unequally sized groups. There were four control groups, control 1 was intact, control 2 olive oil, control 3 carbon tetrachloride diluted with olive oil at a ratio of 3:1 and six treatment groups was injected into the muscle between dorsal fin and lateral line of fish with a dose of 1ml / kg of fish weight in the remaining 6 aquariums. After 24 hours from injection, doses of 100 and 200 ml / kg of fish weight from each extracts were injected into treatment groups. This was repeated 6 times every 48 hours. Then, blood samples were collected from caudal fin to measure liver enzymes (ALP, AST, and ALT), TG and cholesterol. Data were analyzed by SPSS. The standard deviation turned out to be significant (P≤0.05).
Results: The results showed a remarkable increase in liver enzymes and TG as well as cholesterol and histopathological studies in the case group of carbon tetrachloride compared to the control group. After the injection of extracts, a significant decrease (p≤0.05) was observed in the liver enzymes and triglyceride and cholesterol levels. The greatest effect in reducing liver enzymes, cholesterol and triglycerides was related to Silybum marianum at a dose of 200 mg / kg
Conclusion: The Ficcus carica extract and Spirulina platensis in many cases are close to Silybum marianum, which shows the importance of their effect in the treatment and prevention of fatty liver. Regarding blood factors, the greatest effect in treating the toxic effects of carbon tetrachloride is related to fig extract, then Silybum marianum and then Spirulina platensis